利用western blot、竞争性ELISA、细菌分离和全基因组测序对某2400头商业母猪养殖场猪布鲁氏菌血清学假阳性小肠结肠炎耶尔森菌O:9的诊断

IF 0.7 4区农林科学 Q3 Agricultural and Biological Sciences

Journal of Swine Health and Production Pub Date : 2020-01-01 DOI:10.54846/jshap/1153

Ross Free, Mark Ladd, R. Capsel, L. Cox, J. Hicks, K. Lantz, Michael J. Neault, James M. Kittrell, B. J. Meade

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引用次数: 1

摘要

尽管美国商品猪的猪布鲁氏菌病已被根除，但猪布鲁氏菌仍然存在于野猪中。因此，开展布鲁氏菌病监测是为了发现和消除任何从野猪向家猪传播的疾病。由于猪布鲁氏菌病的血清学特异性不完善，出现假阳性血清学反应(FPSRs)，必须排除真正的布鲁氏菌感染。在本病例报告中，我们详细介绍了在商业母猪群中使用其他诊断方法排除猪B型感染的过程，包括细菌培养、全基因组测序、western blot和竞争性酶联免疫吸附试验。确定小肠结肠炎耶尔森菌血清型O:9引起FPSRs。

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Diagnosis of Yersinia enterocolitica serovar O:9 in a commercial 2400-sow farm with false-positive Brucella suis serology using western blot, competitive ELISA, bacterial isolation, and whole genome sequencing

Despite eradication of swine brucellosis from US commercial swine, Brucella suis still exists in feral swine. Therefore, brucellosis surveillance occurs to detect and eliminate any disease introduction from feral swine to domestic swine. As serology for swine brucellosis has imperfect specificity, false-positive serological reactions (FPSRs) occur and true brucellosis infection must be ruled out. In this case report, we detail a process to rule out B suis infection in a commercial sow herd using additional diagnostics including bacterial culture, whole genome sequencing, western blot, and competitive enzyme-linked immunosorbent assay. It was determined Yersinia enterocolitica serovar O:9 caused the FPSRs.

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来源期刊

Journal of Swine Health and Production 农林科学-兽医学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Journal of Swine Health & Production (JSHAP) is an open-access and peer-reviewed journal published by the American Association of Swine Veterinarians (AASV) since 1993. The aim of the journal is the timely publication of peer-reviewed papers with a scope that encompasses the many domains of applied swine health and production, including the diagnosis, treatment, management, prevention and eradication of swine diseases, welfare & behavior, nutrition, public health, epidemiology, food safety, biosecurity, pharmaceuticals, antimicrobial use and resistance, reproduction, growth, systems flow, economics, and facility design. The journal provides a platform for researchers, veterinary practitioners, academics, and students to share their work with an international audience. The journal publishes information that contains an applied and practical focus and presents scientific information that is accessible to the busy veterinary practitioner as well as to the research and academic community. Hence, manuscripts with an applied focus are considered for publication, and the journal publishes original research, brief communications, case reports/series, literature reviews, commentaries, diagnostic notes, production tools, and practice tips. All manuscripts submitted to the Journal of Swine Health & Production are peer-reviewed.