{"title":"获得性免疫缺陷综合征合并肺部感染患者痰液、血液和支气管肺泡灌洗液中病原体的检出率","authors":"Ya Tian, Yu Wang, Yajie Wang, Chen Chen, Zhen Chen, Hui-zhu Wang, Fujie Zhang","doi":"10.3760/CMA.J.ISSN.1000-6680.2019.06.005","DOIUrl":null,"url":null,"abstract":"Objective \nTo study the detection rate of pathogens from sputum, blood, and bronchoalveolar lavage fluid (BALF) samples in acquired immunodeficiency syndrome (AIDS) patients complicated with pulmonary infection. \n \n \nMethods \nSeventy-three hospitalized AIDS patients complicated with pulmonary infection in Beijing Ditan Hospital, Capital Medical University were enrolled from February 2018 to September 2018. Blood, sputum and BALF samples were collected. Blood samples were cultured to detect anaerobic bacteria, aerobic bacteria, fungi and mycobacteria. Antigen agglutination method was applied in blood samples to detect cryptococcus neoformans. The sputum samples were tested for Mycobacterium tuberculosis by acid-fast staining and were cultured to detect bacteria and fungi. The sputum samples were observed under microscope for sporotrichosis and fungal spores. The BALF samples were cultured to detect bacteria and fungi. The BALF samples were tested for Mycobacterium tuberculosis by polymerase chain reaction amplification and acid-fast staining. Pneumocystis were detected in BALF samples by methenamine silver staining method. The BALF samples were observed under a microscope for sporotrichosis and fungal spores. The detection rate of pathogens from blood, sputum and BALF samples were compared. Chi-square test was conducted for statistical analysis. \n \n \nResults \nIn 73 AIDS patients complicated with pulmonary infection, the pathogen detection rates in blood, sputum and BALF samples were 8 (11.0%), 23 (31.5%) and 48 (65.8%), respectively. The difference was statistically significant (F=48.513, P<0.01). The detection rate in BALF samples was significantly higher than that in blood or sputum samples (χ2=43.349 and 17.136, respectively, both P<0.01). The detection rate in sputum samples was significantly higher than that in blood (χ2=9.215, P<0.05). The highest detection rates of pathogens in blood, sputum and BALF samples were Talaromyces marneffei 4.1%(3), viridans group streptococci 16.4%(12) and 35.6%(26), respectively. \n \n \nConclusions \nThe detection rate of pathogens in BALF samples from AIDS patients complicated with pulmonary infection is the highest, followed by sputum and blood samples. \n \n \nKey words: \nAcquired immunodeficiency syndrome; Lung infection; Etiological diagnosis","PeriodicalId":10127,"journal":{"name":"中华传染病杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection rate of pathogens from sputum, blood, and bronchoalveolar lavage fluid samples in acquired immunodeficiency syndrome patients complicated with pulmonary infection\",\"authors\":\"Ya Tian, Yu Wang, Yajie Wang, Chen Chen, Zhen Chen, Hui-zhu Wang, Fujie Zhang\",\"doi\":\"10.3760/CMA.J.ISSN.1000-6680.2019.06.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo study the detection rate of pathogens from sputum, blood, and bronchoalveolar lavage fluid (BALF) samples in acquired immunodeficiency syndrome (AIDS) patients complicated with pulmonary infection. \\n \\n \\nMethods \\nSeventy-three hospitalized AIDS patients complicated with pulmonary infection in Beijing Ditan Hospital, Capital Medical University were enrolled from February 2018 to September 2018. Blood, sputum and BALF samples were collected. Blood samples were cultured to detect anaerobic bacteria, aerobic bacteria, fungi and mycobacteria. Antigen agglutination method was applied in blood samples to detect cryptococcus neoformans. The sputum samples were tested for Mycobacterium tuberculosis by acid-fast staining and were cultured to detect bacteria and fungi. The sputum samples were observed under microscope for sporotrichosis and fungal spores. The BALF samples were cultured to detect bacteria and fungi. The BALF samples were tested for Mycobacterium tuberculosis by polymerase chain reaction amplification and acid-fast staining. Pneumocystis were detected in BALF samples by methenamine silver staining method. The BALF samples were observed under a microscope for sporotrichosis and fungal spores. The detection rate of pathogens from blood, sputum and BALF samples were compared. Chi-square test was conducted for statistical analysis. \\n \\n \\nResults \\nIn 73 AIDS patients complicated with pulmonary infection, the pathogen detection rates in blood, sputum and BALF samples were 8 (11.0%), 23 (31.5%) and 48 (65.8%), respectively. The difference was statistically significant (F=48.513, P<0.01). The detection rate in BALF samples was significantly higher than that in blood or sputum samples (χ2=43.349 and 17.136, respectively, both P<0.01). The detection rate in sputum samples was significantly higher than that in blood (χ2=9.215, P<0.05). The highest detection rates of pathogens in blood, sputum and BALF samples were Talaromyces marneffei 4.1%(3), viridans group streptococci 16.4%(12) and 35.6%(26), respectively. \\n \\n \\nConclusions \\nThe detection rate of pathogens in BALF samples from AIDS patients complicated with pulmonary infection is the highest, followed by sputum and blood samples. \\n \\n \\nKey words: \\nAcquired immunodeficiency syndrome; Lung infection; Etiological diagnosis\",\"PeriodicalId\":10127,\"journal\":{\"name\":\"中华传染病杂志\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华传染病杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1000-6680.2019.06.005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华传染病杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1000-6680.2019.06.005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection rate of pathogens from sputum, blood, and bronchoalveolar lavage fluid samples in acquired immunodeficiency syndrome patients complicated with pulmonary infection
Objective
To study the detection rate of pathogens from sputum, blood, and bronchoalveolar lavage fluid (BALF) samples in acquired immunodeficiency syndrome (AIDS) patients complicated with pulmonary infection.
Methods
Seventy-three hospitalized AIDS patients complicated with pulmonary infection in Beijing Ditan Hospital, Capital Medical University were enrolled from February 2018 to September 2018. Blood, sputum and BALF samples were collected. Blood samples were cultured to detect anaerobic bacteria, aerobic bacteria, fungi and mycobacteria. Antigen agglutination method was applied in blood samples to detect cryptococcus neoformans. The sputum samples were tested for Mycobacterium tuberculosis by acid-fast staining and were cultured to detect bacteria and fungi. The sputum samples were observed under microscope for sporotrichosis and fungal spores. The BALF samples were cultured to detect bacteria and fungi. The BALF samples were tested for Mycobacterium tuberculosis by polymerase chain reaction amplification and acid-fast staining. Pneumocystis were detected in BALF samples by methenamine silver staining method. The BALF samples were observed under a microscope for sporotrichosis and fungal spores. The detection rate of pathogens from blood, sputum and BALF samples were compared. Chi-square test was conducted for statistical analysis.
Results
In 73 AIDS patients complicated with pulmonary infection, the pathogen detection rates in blood, sputum and BALF samples were 8 (11.0%), 23 (31.5%) and 48 (65.8%), respectively. The difference was statistically significant (F=48.513, P<0.01). The detection rate in BALF samples was significantly higher than that in blood or sputum samples (χ2=43.349 and 17.136, respectively, both P<0.01). The detection rate in sputum samples was significantly higher than that in blood (χ2=9.215, P<0.05). The highest detection rates of pathogens in blood, sputum and BALF samples were Talaromyces marneffei 4.1%(3), viridans group streptococci 16.4%(12) and 35.6%(26), respectively.
Conclusions
The detection rate of pathogens in BALF samples from AIDS patients complicated with pulmonary infection is the highest, followed by sputum and blood samples.
Key words:
Acquired immunodeficiency syndrome; Lung infection; Etiological diagnosis