In order to understand the toxic effects of Sb (antimony) on soil invertebrates and compare the differences in Sb toxicity in different types of soil, three groups of individual level evaluation indicators, namely mortality rate, escape rate, and reproduction number, were selected to study the acute and chronic toxic effects of exogenous Sb addition on the model organism, Folsomia candida, in three typical soils (Helen black soil, Qiyang red soil, and Beijing tidal soil). The results showed that:, Based on the measured w (Sb total), the 2d-EC50 (EC50 is the half effective concentration) of Sb affecting the escape of fleas in the above three soils were 298,>431 [higher than the highest w (Sb total) in the soil] and 132 mg/kg, respectively; The 7 d-LC50 (LC50 is the median lethal concentration) that affects the death of jumping insects are 3352, 4007, and 2105 mg/kg, respectively; The 28 d-LC50 that affects the death of fleas were 2271, 1865, and 703 mg/kg, respectively, and the 28 d-EC50 that affects the reproduction of fleas was 1799, 1323, and 307 mg/kg, respectively. From the above toxicity thresholds, it can be seen that the sensitivity of flea escape rate is higher than that of mortality rate and reproduction number. There is a significant difference in the toxicity of Sb to fleas in different soils. The toxicity of Sb to fleas in Beijing Chao soil is close to six times higher than that in Helen black soil and Qiyang red soil, It indicates that different soil physicochemical properties have a significant impact on the ecotoxicity effect of Sb. However, the difference in toxicity thresholds of Sb in the above three soils obtained based on w (Sb water extraction) decreases, indicating a significant correlation between water extracted Sb and its toxicity, which can better explain the differences in Sb toxicity among different soils. The research results can provide a basis for establishing a toxicity prediction model for Sb in soil in China and formulating quality standard values for Sb