纳米银颗粒胰蛋白酶电晕形成及其对酶效的影响

IF 1.4 Q3 CHEMISTRY, MULTIDISCIPLINARY
Khorshid Darvishi-Ganji, Fateme Mirzajani, A. Aliahmadi, Alireza Fakhari-Zavareh, A. Ghassempour
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引用次数: 1

摘要

本文研究了球形金属纳米粒子上蛋白质电晕的形成,探讨了银纳米粒子(AgNPs)对蛋白质活性和构象的可能影响。在标准酶解条件下,在没有和存在不同浓度AgNPs的情况下,监测胰蛋白酶对人血清白蛋白(HSA)的消化能力。对酶与HSA的比值、纳米颗粒处理时间和温度进行了评价。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)和纳米液相色谱电喷雾(离子阱)质谱(nano LC-ESI/MS、LC-ESI/MS)研究处理后胰蛋白酶分子以硬冠(HC)和软冠(SC)形式存在的活性。此外,利用紫外/可见光谱(UV-Vis)、动态光散射(DLS)和荧光分光光度法监测了银纳米颗粒的特性和纳米颗粒表面HSA电晕的形成。结果表明,除了电晕的形成外,AgNPs/胰蛋白酶的相互作用也降低了胰蛋白酶的水解能力。此外,AgNPs/HSA的相遇会影响纳米颗粒和HSA的分子特征。结合荧光研究,表明HSA二级结构。LC-ESI/MS数据显示,受影响最大的HSA颗粒具有α-螺旋结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Silver Nanoparticle Trypsin Corona Formation and the Impacts on Enzymatic Potency
Herein, the protein corona formation on the spherical metal nanoparticles was studied to investigate the possible effects of silver nanoparticles (AgNPs) on the protein activity and conformation. The digestion capability of trypsin was monitored on the human serum albumin (HSA) at standard enzymatic hydrolysis conditions in the absence and presence of different concentrations of AgNPs. So the ratio of enzyme:HSA, the duration and the temperature of nanoparticle treatment were evaluated. The activity of treated trypsin molecules, in the form of hard (HC) and soft corona (SC) were studied using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE), and nano liquid chromatography electrospray (ion trap) mass spectrometry (nano LC-ESI/MS, LC-ESI/MS). In addition the characteristics of silver nanoparticles and the formation of HSA corona on the nanoparticle surface were monitored using ultra-violet/visible spectroscopy (UV-Vis), Dynamic light scattering (DLS) and fluorescence spectrophotometry. The results demonstrated that not only the corona formation but also the AgNPs/Trypsin interaction, decreases the hydrolysis potency of trypsin. Furthermore, the encountering of the AgNPs/HSA could influence both nanoparticles and HSA molecule features. Accompanied with fluorescence study, that the HSA secondary structure. Also LC-ESI/MS data revealed the most affected HSA triptics have α-helix structure.
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来源期刊
Physical Chemistry Research
Physical Chemistry Research CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
2.70
自引率
8.30%
发文量
18
期刊介绍: The motivation for this new journal is the tremendous increasing of useful articles in the field of Physical Chemistry and the related subjects in recent years, and the need of communication between Physical Chemists, Physicists and Biophysicists. We attempt to establish this fruitful communication and quick publication. High quality original papers in English dealing with experimental, theoretical and applied research related to physics and chemistry are welcomed. This journal accepts your report for publication as a regular article, review, and Letter. Review articles discussing specific areas of physical chemistry of current chemical or physical importance are also published. Subjects of Interest: Thermodynamics, Statistical Mechanics, Statistical Thermodynamics, Molecular Spectroscopy, Quantum Chemistry, Computational Chemistry, Physical Chemistry of Life Sciences, Surface Chemistry, Catalysis, Physical Chemistry of Electrochemistry, Kinetics, Nanochemistry and Nanophysics, Liquid Crystals, Ionic Liquid, Photochemistry, Experimental article of Physical chemistry. Mathematical Chemistry.
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