渗漏肠细胞培养模型(Caco-2/THP-1共培养)对替代蛋白质来源给药的反应

M. Marzorati, P. van den Abbeele, L. Verstrepen, Jelle De Medts, R. Ekmay
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引用次数: 1

摘要

已经出现了几种替代蛋白质,它们可能会改善我们食品系统的环境足迹。对这些蛋白质来源对胃肠道健康的影响的评估是有限的。进行了一项研究,以确定传统和替代膳食蛋白质来源的水提取物是否对渗漏的肠道细胞培养模型有不同的影响。将豆粕、鱼粉、贾迪尼Cyberlindera jadinii、Saccharomyces sp.、Bio-Mos、蛋白核小球藻、外突甲基杆菌、大肠杆菌和发光菌的水提取物施用到Caco-2/THP-1共培养物上,并测定跨上皮电阻(TEER)和IL-1β、IL-6、IL-8、IL-10、TNF-α、CXCL10和MCP-1浓度,κB活性。进行主成分分析和K均值聚类。确定了三个聚类:一个用于豆粕,一个用于细菌餐,一个针对其余来源。菌粉簇表现出促炎特性,即与TNF-α、IL-1β、IL-8和NF-κB相关。豆粕簇表现出促炎和抗炎特性,而含有剩余蛋白质的第三簇表现出抗炎特性(与TEER和IL-10相关)。这些结果表明,在渗漏肠道模型中,酵母蛋白的水提取物对肠道健康的贡献更大,而细菌蛋白的贡献最小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Response of a Leaky Gut Cell Culture Model (Caco-2/THP-1 Co-Culture) to Administration of Alternative Protein Sources
Several alternative proteins have emerged that may improve the environmental footprint of our food system. Evaluations into the impact of these protein sources on gastrointestinal health is limited. A study was performed to determine whether aqueous extracts from dietary protein sources, both traditional and alternative, had a differential impact on a leaky gut cell culture model. Aqueous extracts of soybean meal, fish meal, Cyberlindnera jadinii, Saccharomyces sp., Bio-Mos, Chlorella pyrenoidosa, Methylobacterium extorquens, Escherichia coli, and Hermetia illucens were administered onto a Caco-2/THP-1 co-culture and the transepithelial electrical resistance (TEER) and IL-1β, IL-6, IL-8, IL-10, TNF-α, CXCL10, and MCP-1 concentrations, and NF-κB activity were determined. Principal components analysis and K means clustering were performed. Three clusters were identified: one for soybean meal, one for bacterial meals, and one for the remaining sources. The bacterial meal cluster exhibited pro-inflammatory properties, i.e., correlated with TNF-α, IL-1β, IL-8, and NF-κB. The soybean meal cluster exhibited both pro- and anti-inflammatory properties, whereas the third cluster containing the remaining proteins exhibited anti-inflammatory properties (correlated with TEER and IL-10). These results suggest that aqueous extracts from yeast proteins contribute more positively, and bacterial proteins contribute the least positively, towards intestinal health in a leaky gut model.
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