“BARC-7”大豆种子蛋白和油脂在两个f2衍生定位群体中的遗传分析

IF 1 Q3 AGRONOMY

Journal of Crop Improvement Pub Date : 2022-02-22 DOI:10.1080/15427528.2022.2033373

F. Ravelombola, P. Chen, T. Vuong, H. Nguyen, R. Mian, A. Acuña, L. Florez‐Palacios, C. Wu, D. Harrison, M. de Oliveira, J. Winter, M. Ford, M. D. da Silva, L. Mozzoni

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引用次数: 1

摘要

大豆（Glycine max（L.）Merr.]种子蛋白遗传已被广泛研究；然而，高蛋白“BARC-7”大豆的遗传机制尚不清楚。在本研究中，我们使用了来自两个大豆群体的250个F2衍生品系进行数量性状基因座（QTL）定位。UA 5814HP是一个常见的亲本，其蛋白质含量高，可追溯到BARC-7作为外祖父。现场实验采用随机完全区块设计，在四个环境中进行一次复制。使用近红外（NIR）仪器对种子蛋白质和油进行定量。使用Infinium Soy6KSNP微珠芯片构建遗传连锁图谱。QTL分析采用复合区间作图法。在第6、13和20号染色体上鉴定了蛋白质和油的QTL。20号染色体上已知的主要QTL未被检测到，但20号染色体下游的一个新QTL（仅在群体2中检测到）具有从BARC-7衍生的亲本遗传的高蛋白等位基因。目前正在进行精细测绘工作，以确认这些结果。

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Genetics of seed protein and oil inherited from “BARC-7” soybean in two F2-derived mapping populations

ABSTRACT Soybean [Glycine max (L.) Merr.] seed protein inheritance has been extensively studied; however, genetics of high-protein “BARC-7” soybean are still unknown. In this study, we used 250 F2-derived lines from each of two soybean populations for quantitative trait loci (QTL) mapping. UA 5814HP, with high-protein content, tracing to BARC-7 as maternal grandfather, was a common parent. Field experiments were conducted using a randomized complete block design with one replication across four environments. Seed protein and oil were quantified using near-infrared (NIR) instrument. Genetic linkage maps were constructed using the Infinium Soy6KSNP Beadchips. QTL analysis was performed using a composite interval mapping method. QTL for protein and oil were identified on chromosomes 6, 13, and 20. The known major QTL on chromosome 20 was not detected, but a novel QTL further downstream on chromosome 20 (only detected in population two) had high-protein alleles inherited from BARC-7-derived parent. Fine mapping efforts are currently ongoing for confirmation of these results.

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来源期刊

Journal of Crop Improvement Multiple-

CiteScore

3.30

自引率

7.70%

发文量

期刊介绍： Journal of Crop Science and Biotechnology (JCSB) is a peer-reviewed international journal published four times a year. JCSB publishes novel and advanced original research articles on topics related to the production science of field crops and resource plants, including cropping systems, sustainable agriculture, environmental change, post-harvest management, biodiversity, crop improvement, and recent advances in physiology and molecular biology. Also covered are related subjects in a wide range of sciences such as the ecological and physiological aspects of crop production and genetic, breeding, and biotechnological approaches for crop improvement.