{"title":"公羊精液液体储存:相关损害及改进","authors":"Serge Leugoué Kameni, F. Meutchieye, F. Ngoula","doi":"10.4236/ojas.2021.113033","DOIUrl":null,"url":null,"abstract":"The successful application of assisted reproductive techniques (ARTs) in \novine as in other mammal species relies on many factors among which the quality \nof the semen used. After collection, semen samples are generally processed for \nstorage (liquid storage or cryoconservation) before being used for insemination \nor in vitro embryo \nproduction. During the liquid storage process, sperm cells are exposed to \nartificial conditions which lead to oxidative stress—the imbalance between \npro-oxidants and antioxidants (AO), following overproduction of reactive oxygen \nspecies (ROS)—resulting in ultrastructural, biochemical \nand functional damages of spermatozoa. Especially, viability, motility, \nmitochondrial activity, membrane integrity, and acrosome integrity are reduced while morphological \nabnormalities, DNA fragmentation, and lipid peroxidation (LPO) are increased, \naffecting the fertilizing ability and subsequent early embryonic development \nwhen using standard extenders. Indeed, an optimal semen extender must not only \nregulate and support an environment of adequate pH and buffering capacity to \nprotect spermatozoa from osmotic and cooling stresses, but, also prevent the \ngeneration and/or scavenge excess ROS. To improve ram semen liquid storage, \nseveral methods have been developed with the supplementation of extenders with \nantioxidants or antioxidant like-compounds (enzymes, amino-acids, vitamins, \nplant extracts), seminal plasma, sugars, fatty acids, and nanoparticles being a \nrelevant approach. Promising results have been registered with the \nsupplementation of extenders with these compounds, confirming they can be used \nto preserve ram semen quality and fertility. Therefore, the present review \nprovides an updated overview of the damages and associated mechanisms that ram \nspermatozoa undergo during liquid storage. Moreover, the supplementation of \nextenders with different compounds as a tool to improve semen storage is also \ndiscussed as well as their efficiency to reduce and/or prevent sperm damages \nduring storage.","PeriodicalId":62784,"journal":{"name":"动物科学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Liquid Storage of Ram Semen: Associated Damages and Improvement\",\"authors\":\"Serge Leugoué Kameni, F. Meutchieye, F. Ngoula\",\"doi\":\"10.4236/ojas.2021.113033\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The successful application of assisted reproductive techniques (ARTs) in \\novine as in other mammal species relies on many factors among which the quality \\nof the semen used. After collection, semen samples are generally processed for \\nstorage (liquid storage or cryoconservation) before being used for insemination \\nor in vitro embryo \\nproduction. During the liquid storage process, sperm cells are exposed to \\nartificial conditions which lead to oxidative stress—the imbalance between \\npro-oxidants and antioxidants (AO), following overproduction of reactive oxygen \\nspecies (ROS)—resulting in ultrastructural, biochemical \\nand functional damages of spermatozoa. Especially, viability, motility, \\nmitochondrial activity, membrane integrity, and acrosome integrity are reduced while morphological \\nabnormalities, DNA fragmentation, and lipid peroxidation (LPO) are increased, \\naffecting the fertilizing ability and subsequent early embryonic development \\nwhen using standard extenders. Indeed, an optimal semen extender must not only \\nregulate and support an environment of adequate pH and buffering capacity to \\nprotect spermatozoa from osmotic and cooling stresses, but, also prevent the \\ngeneration and/or scavenge excess ROS. To improve ram semen liquid storage, \\nseveral methods have been developed with the supplementation of extenders with \\nantioxidants or antioxidant like-compounds (enzymes, amino-acids, vitamins, \\nplant extracts), seminal plasma, sugars, fatty acids, and nanoparticles being a \\nrelevant approach. Promising results have been registered with the \\nsupplementation of extenders with these compounds, confirming they can be used \\nto preserve ram semen quality and fertility. Therefore, the present review \\nprovides an updated overview of the damages and associated mechanisms that ram \\nspermatozoa undergo during liquid storage. Moreover, the supplementation of \\nextenders with different compounds as a tool to improve semen storage is also \\ndiscussed as well as their efficiency to reduce and/or prevent sperm damages \\nduring storage.\",\"PeriodicalId\":62784,\"journal\":{\"name\":\"动物科学期刊(英文)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"动物科学期刊(英文)\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://doi.org/10.4236/ojas.2021.113033\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"动物科学期刊(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.4236/ojas.2021.113033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Liquid Storage of Ram Semen: Associated Damages and Improvement
The successful application of assisted reproductive techniques (ARTs) in
ovine as in other mammal species relies on many factors among which the quality
of the semen used. After collection, semen samples are generally processed for
storage (liquid storage or cryoconservation) before being used for insemination
or in vitro embryo
production. During the liquid storage process, sperm cells are exposed to
artificial conditions which lead to oxidative stress—the imbalance between
pro-oxidants and antioxidants (AO), following overproduction of reactive oxygen
species (ROS)—resulting in ultrastructural, biochemical
and functional damages of spermatozoa. Especially, viability, motility,
mitochondrial activity, membrane integrity, and acrosome integrity are reduced while morphological
abnormalities, DNA fragmentation, and lipid peroxidation (LPO) are increased,
affecting the fertilizing ability and subsequent early embryonic development
when using standard extenders. Indeed, an optimal semen extender must not only
regulate and support an environment of adequate pH and buffering capacity to
protect spermatozoa from osmotic and cooling stresses, but, also prevent the
generation and/or scavenge excess ROS. To improve ram semen liquid storage,
several methods have been developed with the supplementation of extenders with
antioxidants or antioxidant like-compounds (enzymes, amino-acids, vitamins,
plant extracts), seminal plasma, sugars, fatty acids, and nanoparticles being a
relevant approach. Promising results have been registered with the
supplementation of extenders with these compounds, confirming they can be used
to preserve ram semen quality and fertility. Therefore, the present review
provides an updated overview of the damages and associated mechanisms that ram
spermatozoa undergo during liquid storage. Moreover, the supplementation of
extenders with different compounds as a tool to improve semen storage is also
discussed as well as their efficiency to reduce and/or prevent sperm damages
during storage.