等离子接枝在聚四氟乙烯薄膜上制备抗菌无毒壳聚糖涂层,用于医疗器械

IF 2.3 4区 材料科学 Q2 Chemistry
Juliana M. Vaz, Thiago B. Taketa, Jacobo Hernandez-Montelongo, Larissa M. C. G. Fiúza, Cristiano Rodrigues, Marisa M. Beppu, Rodrigo S. Vieira
{"title":"等离子接枝在聚四氟乙烯薄膜上制备抗菌无毒壳聚糖涂层,用于医疗器械","authors":"Juliana M. Vaz,&nbsp;Thiago B. Taketa,&nbsp;Jacobo Hernandez-Montelongo,&nbsp;Larissa M. C. G. Fiúza,&nbsp;Cristiano Rodrigues,&nbsp;Marisa M. Beppu,&nbsp;Rodrigo S. Vieira","doi":"10.1007/s11998-021-00560-3","DOIUrl":null,"url":null,"abstract":"<div><p>Chitosan is an exciting alternative for the development of coating-surfaces due to its large action spectrum against pathogenic microorganisms. However, to produce a stable coating with effective antibacterial action, a compromise between deacetylation degree (DD) and molecular weight (MW) is essential. Four chitosan samples were characterized regarding Mw and DD and correlated with the minimum and bactericide concentrations against <i>E. coli</i>, <i>P. aeruginosa,</i> and <i>S. aureus</i>. CHI80MW (79.7% DD and 7.0 × 10<sup>5</sup> Da) showed the best antibacterial effect and was selected to functionalize polytetrafluoroethylene (PTFE) surfaces by plasma. CHI80MW was grafted onto the PTFE surfaces using two different spacer molecules: poly(ethylene glycol) bis (carboxymethyl) ether (PEG) and poly(ethylene-alt-maleic anhydride) (PA). PTFE-Plasma-PA-CHI80MW exhibited a coating with more attached chitosan and better antibacterial action if compared to PTFE-Plasma-PEG-CHI80MW: after 8 h, PTFE-Plasma-PEG-CHI80MW presented a bacterial reduction of 25-30% for the three bacterial strains, and PTFE-Plasma-PA-CHI80MW reduced them to 77-90%. Moreover, cytotoxicity tests showed that PTFE-Plasma-PA-CHI80MW samples were compatible with human fibroblasts.</p></div>","PeriodicalId":48804,"journal":{"name":"Journal of Coatings Technology and Research","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2022-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Antibacterial noncytotoxic chitosan coatings on polytetrafluoroethylene films by plasma grafting for medical device applications\",\"authors\":\"Juliana M. Vaz,&nbsp;Thiago B. Taketa,&nbsp;Jacobo Hernandez-Montelongo,&nbsp;Larissa M. C. G. Fiúza,&nbsp;Cristiano Rodrigues,&nbsp;Marisa M. Beppu,&nbsp;Rodrigo S. Vieira\",\"doi\":\"10.1007/s11998-021-00560-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Chitosan is an exciting alternative for the development of coating-surfaces due to its large action spectrum against pathogenic microorganisms. However, to produce a stable coating with effective antibacterial action, a compromise between deacetylation degree (DD) and molecular weight (MW) is essential. Four chitosan samples were characterized regarding Mw and DD and correlated with the minimum and bactericide concentrations against <i>E. coli</i>, <i>P. aeruginosa,</i> and <i>S. aureus</i>. CHI80MW (79.7% DD and 7.0 × 10<sup>5</sup> Da) showed the best antibacterial effect and was selected to functionalize polytetrafluoroethylene (PTFE) surfaces by plasma. CHI80MW was grafted onto the PTFE surfaces using two different spacer molecules: poly(ethylene glycol) bis (carboxymethyl) ether (PEG) and poly(ethylene-alt-maleic anhydride) (PA). PTFE-Plasma-PA-CHI80MW exhibited a coating with more attached chitosan and better antibacterial action if compared to PTFE-Plasma-PEG-CHI80MW: after 8 h, PTFE-Plasma-PEG-CHI80MW presented a bacterial reduction of 25-30% for the three bacterial strains, and PTFE-Plasma-PA-CHI80MW reduced them to 77-90%. Moreover, cytotoxicity tests showed that PTFE-Plasma-PA-CHI80MW samples were compatible with human fibroblasts.</p></div>\",\"PeriodicalId\":48804,\"journal\":{\"name\":\"Journal of Coatings Technology and Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Coatings Technology and Research\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11998-021-00560-3\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Chemistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Coatings Technology and Research","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s11998-021-00560-3","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Chemistry","Score":null,"Total":0}
引用次数: 2

摘要

由于壳聚糖对病原微生物的作用谱大,它是发展涂层表面的一个令人兴奋的选择。然而,为了制备出具有有效抗菌作用的稳定涂层,脱乙酰度(DD)和分子量(MW)之间的折衷是必不可少的。对4种壳聚糖样品进行了分子量和DD的表征,并与对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的最低抑菌浓度和抑菌浓度相关。CHI80MW (79.7% DD和7.0 × 105 Da)的抗菌效果最好,并被选择用于等离子体功能化聚四氟乙烯(PTFE)表面。CHI80MW用两种不同的间隔分子:聚乙二醇双羧甲基醚(PEG)和聚乙烯-马来酸酐(PA)接枝到聚四氟乙烯表面。与PTFE-Plasma-PA-CHI80MW相比,PTFE-Plasma-PA-CHI80MW涂层具有更多的壳聚糖和更好的抗菌作用:8 h后,PTFE-Plasma-PEG-CHI80MW对三种细菌的细菌数量减少了25-30%,而PTFE-Plasma-PA-CHI80MW则减少了77-90%。此外,细胞毒性试验表明,PTFE-Plasma-PA-CHI80MW样品与人成纤维细胞相容。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial noncytotoxic chitosan coatings on polytetrafluoroethylene films by plasma grafting for medical device applications

Chitosan is an exciting alternative for the development of coating-surfaces due to its large action spectrum against pathogenic microorganisms. However, to produce a stable coating with effective antibacterial action, a compromise between deacetylation degree (DD) and molecular weight (MW) is essential. Four chitosan samples were characterized regarding Mw and DD and correlated with the minimum and bactericide concentrations against E. coli, P. aeruginosa, and S. aureus. CHI80MW (79.7% DD and 7.0 × 105 Da) showed the best antibacterial effect and was selected to functionalize polytetrafluoroethylene (PTFE) surfaces by plasma. CHI80MW was grafted onto the PTFE surfaces using two different spacer molecules: poly(ethylene glycol) bis (carboxymethyl) ether (PEG) and poly(ethylene-alt-maleic anhydride) (PA). PTFE-Plasma-PA-CHI80MW exhibited a coating with more attached chitosan and better antibacterial action if compared to PTFE-Plasma-PEG-CHI80MW: after 8 h, PTFE-Plasma-PEG-CHI80MW presented a bacterial reduction of 25-30% for the three bacterial strains, and PTFE-Plasma-PA-CHI80MW reduced them to 77-90%. Moreover, cytotoxicity tests showed that PTFE-Plasma-PA-CHI80MW samples were compatible with human fibroblasts.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Coatings Technology and Research
Journal of Coatings Technology and Research CHEMISTRY, APPLIED-MATERIALS SCIENCE, COATINGS & FILMS
CiteScore
4.40
自引率
8.70%
发文量
0
期刊介绍: Journal of Coatings Technology and Research (JCTR) is a forum for the exchange of research, experience, knowledge and ideas among those with a professional interest in the science, technology and manufacture of functional, protective and decorative coatings including paints, inks and related coatings and their raw materials, and similar topics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信