Pumilio rna结合家族成员1在胰腺癌血管生成中的促进作用

IF 2.6 4区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Canadian Journal of Gastroenterology and Hepatology Pub Date : 2022-12-14 eCollection Date: 2022-01-01 DOI:10.1155/2022/9202531
Haisu Dai, Yan Jiang, Zhipeng Liu, Xingxing Su, Yishi Yang, Zhiyu Chen
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引用次数: 0

摘要

我们前期研究发现Pumilio rna结合家族成员1 (PUM1)基因在胰腺癌(PC)组织中异常表达,其敲低可抑制PC细胞的生长和转移。在此,我们旨在进一步研究其在血管生成中的作用。免疫组化分析CD31和PUM1在PC组织和皮下异种移植肿瘤中的表达水平。PC组织中的CD31水平以微血管密度(MVD)表达。MVD值与PUM1蛋白表达呈正相关。PUM1在PC细胞系中成功过表达或沉默。与过表达PUM1的PC细胞共培养后,HUVECs的增殖、迁移、侵袭和成管能力增强。在PC细胞中,PUM1过表达增加细胞外和细胞内VEGFA蛋白水平。此外,当HUVECs与过表达PUM1的PC细胞共培养时,HUVECs中血管生成相关的信号被激活。然而,用PUM1沉默的PC细胞却有相反的效果。此外,过表达PUM1的皮下异种移植物肿瘤CD31的表达水平较高,而沉默PUM1的皮下异种移植物肿瘤CD31的表达水平较低。由此可见,PC细胞中的PUM1可能在PC血管生成中起促进作用。PUM1可能是PC细胞血管生成的新调控因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pumilio RNA-Binding Family Member 1 Plays a Promoting Role on Pancreatic Cancer Angiogenesis.

Our previous studies showed that Pumilio RNA-binding family member 1 (PUM1) gene is abnormally expressed in pancreatic cancer (PC) tissues, and its knockdown suppresses the growth and metastasis of PC cells. Here, we aimed to further investigate its role in angiogenesis. Immunohistochemical assays were carried out to analyze CD31 and PUM1 expression levels in PC tissues and in subcutaneous xenograft tumors. CD31 levels in PC tissues are expressed as microvessel density (MVD). MVD value was positively correlated with PUM1 protein expression. PUM1 was successfully overexpressed or silenced in the PC cell lines. The proliferation, migration, invasion, and tube formation ability of HUVECs were enhanced when cocultured with PC cells overexpressing PUM1. PUM1 overexpression increased extracellular and intracellular VEGFA protein levels in PC cells. Moreover, angiogenesis-related signaling in HUVECs was activated when HUVECs were cocultured with PC cells overexpressing PUM1. Nevertheless, PC cells silenced with PUM1 had the opposite effect. Moreover, subcutaneous xenograft tumors overexpressing PUM1 have the higher expression level of CD31, while subcutaneous xenograft tumors silencing PUM1 have the lower expression level of CD31. In conclusion, PUM1 in PC cells may play a promoting role in PC angiogenesis. PUM1 may be a new regulator of angiogenesis in PC cells.

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来源期刊
自引率
0.00%
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90
期刊介绍: Canadian Journal of Gastroenterology and Hepatology is a peer-reviewed, open access journal that publishes original research articles, review articles, and clinical studies in all areas of gastroenterology and liver disease - medicine and surgery. The Canadian Journal of Gastroenterology and Hepatology is sponsored by the Canadian Association of Gastroenterology and the Canadian Association for the Study of the Liver.
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