Devvret Verma, Neema Tufchi, Kumud Pant, A. Thapliyal
{"title":"豌豆抗氧化蛋白的计算机分析和同源性建模","authors":"Devvret Verma, Neema Tufchi, Kumud Pant, A. Thapliyal","doi":"10.5530/ctbp.2020.2.12","DOIUrl":null,"url":null,"abstract":"In-silico analysis and homology modelling of Antioxidant Proteins Abstract Garden Peas are edible seeds that have an essential nutrient required for human diet. Peas contain phytochemicals that shows antioxidant activities. A computational approach was adopted to analyse the characteristic properties and structure of the Peas antioxidant proteins. Superoxidase dismutase (SOD), catalase, phospholipid hydroperoxide g lutathione p eroxidase-like p rotein (PHGPX) and ascorbate peroxidase (APX) are the antioxidant proteins which have been used in this study. Physiochemical properties such as isoelectric point, molecular weight, aliphatic index etc. were analysed by using the Expasy’s Prot Param server. Secondary structure of these proteins was analysed to study the functional characteristic of the protein. The crystal structure of the ascorbate peroxidase was available, but the crystal structures of other proteins were not available in any of the protein structure database. Hence the threedimensional structures of these proteins were generated using Swiss Modeller and Geno3D followed by validation through SAVES server. This analysis will provide additional help for the analysis of crystal structure for further experimentation.","PeriodicalId":10980,"journal":{"name":"Current Trends in Biotechnology and Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In-silico analysis and homology modelling of antioxidant proteins present in Pisum sativum\",\"authors\":\"Devvret Verma, Neema Tufchi, Kumud Pant, A. Thapliyal\",\"doi\":\"10.5530/ctbp.2020.2.12\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In-silico analysis and homology modelling of Antioxidant Proteins Abstract Garden Peas are edible seeds that have an essential nutrient required for human diet. Peas contain phytochemicals that shows antioxidant activities. A computational approach was adopted to analyse the characteristic properties and structure of the Peas antioxidant proteins. Superoxidase dismutase (SOD), catalase, phospholipid hydroperoxide g lutathione p eroxidase-like p rotein (PHGPX) and ascorbate peroxidase (APX) are the antioxidant proteins which have been used in this study. Physiochemical properties such as isoelectric point, molecular weight, aliphatic index etc. were analysed by using the Expasy’s Prot Param server. Secondary structure of these proteins was analysed to study the functional characteristic of the protein. The crystal structure of the ascorbate peroxidase was available, but the crystal structures of other proteins were not available in any of the protein structure database. Hence the threedimensional structures of these proteins were generated using Swiss Modeller and Geno3D followed by validation through SAVES server. This analysis will provide additional help for the analysis of crystal structure for further experimentation.\",\"PeriodicalId\":10980,\"journal\":{\"name\":\"Current Trends in Biotechnology and Pharmacy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-07-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Trends in Biotechnology and Pharmacy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5530/ctbp.2020.2.12\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Trends in Biotechnology and Pharmacy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5530/ctbp.2020.2.12","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
In-silico analysis and homology modelling of antioxidant proteins present in Pisum sativum
In-silico analysis and homology modelling of Antioxidant Proteins Abstract Garden Peas are edible seeds that have an essential nutrient required for human diet. Peas contain phytochemicals that shows antioxidant activities. A computational approach was adopted to analyse the characteristic properties and structure of the Peas antioxidant proteins. Superoxidase dismutase (SOD), catalase, phospholipid hydroperoxide g lutathione p eroxidase-like p rotein (PHGPX) and ascorbate peroxidase (APX) are the antioxidant proteins which have been used in this study. Physiochemical properties such as isoelectric point, molecular weight, aliphatic index etc. were analysed by using the Expasy’s Prot Param server. Secondary structure of these proteins was analysed to study the functional characteristic of the protein. The crystal structure of the ascorbate peroxidase was available, but the crystal structures of other proteins were not available in any of the protein structure database. Hence the threedimensional structures of these proteins were generated using Swiss Modeller and Geno3D followed by validation through SAVES server. This analysis will provide additional help for the analysis of crystal structure for further experimentation.
期刊介绍:
The Association of Biotechnology and Pharmacy (ABAP) will be useful to form a forum for scientists so that they can bring together to discuss and find scientific solutions to the problems of society. The annual meetings will help the members to share their knowledge and publish their research knowledge particularly by members and fellows of the Association and special care will be taken to provide an opportunity for young scientists. Besides this the association is planned to organize symposia, seminars and workshops on current developments of Biotechnology and Pharmacy particularly on the subject of current scientific interest, and the proceedings of which will be published regularly. And in view of the vast development of science and to disseminate the problems in publication of research work, an international journal of Current Trends in Biotechnology and Pharmacy has been started by ABAP.