豌豆抗氧化蛋白的计算机分析和同源性建模

Q3 Pharmacology, Toxicology and Pharmaceutics
Devvret Verma, Neema Tufchi, Kumud Pant, A. Thapliyal
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引用次数: 0

摘要

抗氧化蛋白质的计算机分析和同源性建模摘要豌豆是可食用的种子,具有人类饮食所需的基本营养。豌豆中含有具有抗氧化活性的植物化学物质。采用计算方法对豌豆抗氧化蛋白的特性和结构进行了分析。超氧化物歧化酶(SOD)、过氧化氢酶、磷脂氢过氧化物-谷胱甘肽-过氧化酶样p蛋白(PHGPX)和抗坏血酸过氧化物酶(APX)是本研究中使用的抗氧化蛋白。利用Expasy的Prot-Param服务器分析了等电点、分子量、脂肪指数等理化性质。对这些蛋白质的二级结构进行了分析,以研究蛋白质的功能特性。抗坏血酸过氧化物酶的晶体结构是可用的,但其他蛋白质的晶体结构在任何蛋白质结构数据库中都不可用。因此,使用Swiss Modeller和Geno3D生成这些蛋白质的三维结构,然后通过SAVES服务器进行验证。该分析将为进一步实验的晶体结构分析提供额外的帮助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-silico analysis and homology modelling of antioxidant proteins present in Pisum sativum
In-silico analysis and homology modelling of Antioxidant Proteins Abstract Garden Peas are edible seeds that have an essential nutrient required for human diet. Peas contain phytochemicals that shows antioxidant activities. A computational approach was adopted to analyse the characteristic properties and structure of the Peas antioxidant proteins. Superoxidase dismutase (SOD), catalase, phospholipid hydroperoxide g lutathione p eroxidase-like p rotein (PHGPX) and ascorbate peroxidase (APX) are the antioxidant proteins which have been used in this study. Physiochemical properties such as isoelectric point, molecular weight, aliphatic index etc. were analysed by using the Expasy’s Prot Param server. Secondary structure of these proteins was analysed to study the functional characteristic of the protein. The crystal structure of the ascorbate peroxidase was available, but the crystal structures of other proteins were not available in any of the protein structure database. Hence the threedimensional structures of these proteins were generated using Swiss Modeller and Geno3D followed by validation through SAVES server. This analysis will provide additional help for the analysis of crystal structure for further experimentation.
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来源期刊
Current Trends in Biotechnology and Pharmacy
Current Trends in Biotechnology and Pharmacy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.70
自引率
0.00%
发文量
0
期刊介绍: The Association of Biotechnology and Pharmacy (ABAP) will be useful to form a forum for scientists so that they can bring together to discuss and find scientific solutions to the problems of society. The annual meetings will help the members to share their knowledge and publish their research knowledge particularly by members and fellows of the Association and special care will be taken to provide an opportunity for young scientists. Besides this the association is planned to organize symposia, seminars and workshops on current developments of Biotechnology and Pharmacy particularly on the subject of current scientific interest, and the proceedings of which will be published regularly. And in view of the vast development of science and to disseminate the problems in publication of research work, an international journal of Current Trends in Biotechnology and Pharmacy has been started by ABAP.
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