水稻抗洪基因(sub1a)等位基因状态的鉴定

N. Vozhzhova, O. Zhogaleva, A. Dubina, P. Kostylev
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引用次数: 0

摘要

水稻是世界上最重要的农作物之一。水稻作物为亚洲和太平洋地区的许多人提供食物。在俄罗斯,这种作物的生产集中在几个地区,其中之一是罗斯托夫地区。全世界和俄罗斯的农业绿色化趋势正变得越来越稳定。为了使用环境友好的无除草剂技术来对抗杂草,有必要增加同时存活的水稻植株的水层。为了使这一策略发挥作用,有必要开发抗洪水的品种。本研究的目的是鉴定水稻Sub1A抗洪基因的等位基因状态。该研究的对象是90份第六代水稻样本,这些样本是由俄罗斯品种与基因供体品种杂交获得的。采用“DNA-外扩-3”商业试剂盒(俄罗斯Sintol)提取DNA,使用专用诊断标记物Sub1A203进行经典PCR,用2%琼脂糖凝胶电泳分离扩增子,用Bio-Rad ImageLab 6.0.1软件分析电泳图。通过分析,在90份水稻样品中确定了Sub1A基因的等位基因状态。大量样品携带非功能性隐性等位基因,19个样品未确定该基因的存在。在9份样本中,Sub1A基因处于杂合状态。在5个育种样品中鉴定出目的基因的功能性显性等位基因的存在。有人建议将这些样品用于培育抗长期洪水的水稻新品种的育种工作中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of the allelic state of the flood tolerance gene (Sub-1A) in rice breeding samples
Rice is one of the most important crops in the world. Rice crops provide food for many people in Asia and the Pacific. In Russia, the production of this crop is concentrated in several regions, one of which is the Rostov region. The trend towards the greening of agriculture throughout the world and in Russia is becoming more and more stable. In order to combat weeds using an environmentally friendly herbicide-free technology, it is necessary to increase the water layer on simultaneous surviving rice plants. For such a strategy to work, it is necessary to develop varieties that are resistant to flooding. The purpose of the current study was to identify the allelic state of the Sub1A flood resistance gene in breeding rice samples. The objects of the study were 90 rice samples of the sixth generation, identified in hybrids obtained from crossing Russian varieties with gene donor varieties. The analysis was carried out by such methods as DNA extraction using the “DNA-Extran-3” commercial kit (Sintol, Russia), classical PCR with the specialized diagnostic marker Sub1A203, separation of amplicons by electrophoresis in 2 % agarose gels, analysis of electrophoregrams in the software Bio-Rad ImageLab 6.0.1. As a result of the analysis, there was identified the allelic state of the Sub1A gene in 90 rice samples. A significant number of samples carried a non-functional recessive allele, the presence of the gene was not established in 19 samples. The Sub1A gene was in the heterozygous state in 9 samples. The presence of a functional dominant allele of the target gene was identified n 5 breeding samples. There has been recommended to use these samples in breeding work aimed at developing new rice varieties resistant to prolonged flooding.
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