采用单次右侧剖腹手术在猪小肠内进行双简单t型插管

Q3 Agricultural and Biological Sciences
A. Petry, S. Gould, J. Patience
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引用次数: 2

摘要

猪小肠单插管是研究营养物质消化率、向胃肠道输送化合物或重复组织和食糜取样的常用方法。在这方面,它是营养学家和研究人员的重要工具。然而,缺乏使用现代技术描述多种肠道插管的详细方法。本实验的目的是开发一种单一的右侧剖腹手术技术,该技术允许在小肠中插入多个套管,变化最小,并允许在剖腹手术的同一外侧放置两个套管。30头初始体重为30.2±0.78 kg的后备母猪(L337 × Camborough)接受了该手术。每只后备母猪在回肠末端,距回盲瓣约10厘米处安装一个简单的t型套管,在距横结肠后方十二指肠远240厘米处空肠安装第二个t型套管。该手术采用了通常实施的回肠末端插管技术,但修改了开腹位置和套管外置位置,并采用了一种新的方法来确定更近端的套管位置,以减少第二次开腹手术的需要。后备母猪至少恢复7 d,在随后的试验中平均使用67 d。插管寿命从体重30公斤到73公斤不等。实验结束时对猪进行解剖,进行手术部位大体检查和小肠测量。空肠插管平均放置在幽门括约肌远端298.90±9.96 cm处,变异系数为3.33%。因此,该手术提供了一种单一的剖腹手术技术,可以从小肠的多个位置获取食糜,近端插管放置的变化最小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A procedure for dual simple t-cannulation in the small intestine of pigs using a single right flank laparotomy
Singular cannulation in the small intestine of pigs is a common methodology for studying nutrient digestibility, delivering compounds into the gastrointestinal tract, or repeated tissue and digesta sampling. In that respect, it is an important tool for nutritionists and researchers. However, there is a dearth of detailed methodologies describing multiple intestinal cannulations using modern techniques. The objective of this experiment was to develop a single right flank laparotomy technique that allowed for imsertion of multiple cannulas in the small intestine, with minimal variation, and allowed for externalisation of both cannulas on the same lateral side as the laparotomy. Thirty gilts (L337 × Camborough) with an initial body weight of 30.2±0.78 kg underwent the procedure. Each gilt was equipped with a simple t-cannula in the terminal ileum, approximately 10 cm cranial to the ileocecal valve, and a second t-cannula in the jejunum 240 cm distal from where the duodenum is visually posterior to the transverse colon. The procedure used hallmarks of commonly implemented terminal ileal cannulation techniques, but modified the laparotomy location and cannula externalisation sites and used a novel approach for determining the more proximal cannula location to mitigate the need of a second laparotomy. Gilts were allowed to recover for a minimum of 7 d and were used for an average of 67 days in subsequent experimental trials. Cannula longevity was from 30 to 73 kg of body weight. Pigs were necropsied for surgical site gross examination and small intestine measurements at the end of the experiment. The jejunal cannula had a mean placement of 298.90±9.96 cm distal to the pyloric sphincter with a coefficient of variation of 3.33%. Hence, this procedure provided a single laparotomy technique for obtaining digesta from multiple locations of the small intestine, with minimal variation in proximal cannula placement.
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来源期刊
Journal of Applied Animal Nutrition
Journal of Applied Animal Nutrition Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
2.50
自引率
0.00%
发文量
11
审稿时长
10 weeks
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