基于小麻素表现特征的大麻基因型筛选

S. Mishchenko, H. Kyrychenko, I. Laiko
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引用次数: 0

摘要

基于次要(罕见)非精神大麻素含量的大麻采集样本分析,如大麻二氢吡喃(CBC)、大麻二酚(CBDV)和大麻酚(CBN);确定它们与常见化合物之间的相关性;选择有价值的育种基因型。方法。领域,生物化学(大麻素化合物的气相色谱法),结果。对210个不同生态地理和遗传来源(本地和野生型、自交系、杂交种、品种、合成群体、多倍体)的样本进行定量分析,四氢大麻酚(THC)在干燥植物中的含量低于0.08%,显示了该性状的表现水平在气相色谱仪的灵敏度范围内不存在高达0.6838%CBC、0.1719%CBC和0.3274%CBN。在所研究的大麻样品中,CBC的体征与大麻二酚(CBD)含量之间呈中等负相关(r=-0.53),CBC与CBDV含量之间呈弱负相关(r=-0.35),CBC与四氢大麻酚含量呈中等正相关(r=0.57),CBC与CBN含量呈中等呈正相关(r=0.5 9)。次要大麻素化合物的生物合成相当复杂。体征表现受多种遗传和外部因素的影响。偏相关系数(假定三个符号中的一个符号被消除)和多重相关系数(假设一个符号的关系被确定并且另外两个符号被组合)提供了理由来说明CBCA合酶的基因影响CBD的产生,特别是四氢大麻酚。结论。次要大麻素和常见成分之间的线性关系紧密,可以在不存在或低含量精神四氢大麻酸的情况下选择一种或几种化合物含量高的有价值的大麻样品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genotype screening of Cannabis sativa L. based on the specifics of minor cannabinoids manifestation
Analysis of hemp collection samples based on the content of minor (rare) non-psychotropic cannabinoids, such as cannabichromene (CBC), cannabidivarin (CBDV), and cannabinol (CBN); determination of correlation relationships between them and common compounds; selection of valuable breeding genotypes. Methods. Field, biochemical (gas chromatography of cannabinoid compounds), and statistical (pair, partial, and multiple linear correlations). Results. Quantitative analysis of 210 samp­les of various ecological-geographical and genetic origin (local and wild forms, self-filing lines, hybrids, varieties, synthetic populations, polyploids) with a tetrahydrocannabinol (THC) content of less than 0.08% in dried plants showed the level of manifestation of the trait from its absence within the sensitivity of the gas chromatograph up to 0.6838% CBC, 0.1719% CBC and 0.3274% CBN. In the studied hemp samples, a medium negative relationship was found between the signs of the CBC and cannabidiol (CBD) contents (r = –0.53), a weak negative relationship between CBC and CBDV contents (r = –0.35), medium positive relationships between the signs of CBC and THC contents (r = 0.57) and CBC and CBN contents (r = 0.59). A medium positive correlation (r = 0.57) was found between the signs of CBDV and CBD contents, while CBN had a strong positive relationship with THC (r = 0.82). There is almost no correlation between cannabigerol (CBG) and the minor cannabinoids under study. The biosynthesis of minor cannabinoid compounds is quite complex. Signs manifestation is affected by many genetic and external factors. Partial correlation coefficients (given that one of the three signs is eliminated) and multiple correlation coefficients (given that the relationship of one sign is determined and two other signs are combined) give grounds to state that the gene for CBCA-synthase affects the production of CBD and, in particular THC. Conclusions. The closeness of the linear relationships between minor cannabinoids and common components allows selecting valuable hemp samples with a high content of one or several compounds under the absence or low content of psychotropic THC.
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