5-氟尿嘧啶联合氧化锌NPs对人乳腺癌MCF-7细胞的影响:P53基因表达、Bcl-2信号通路和侵袭活性

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
S. Hoseinzadeh, E. Raeisi, Y. Lemoigne, E. Heidarian
{"title":"5-氟尿嘧啶联合氧化锌NPs对人乳腺癌MCF-7细胞的影响:P53基因表达、Bcl-2信号通路和侵袭活性","authors":"S. Hoseinzadeh, E. Raeisi, Y. Lemoigne, E. Heidarian","doi":"10.22038/NMJ.2019.06.000010","DOIUrl":null,"url":null,"abstract":"Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53), p53 gene expression (3.6 folds), reduction of cell invasion (9.82), and plating efficiency (5), thereby leading to the significant reduction of cell viability (40±0.9) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells. Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":"6 1","pages":"232-240"},"PeriodicalIF":1.4000,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity\",\"authors\":\"S. Hoseinzadeh, E. Raeisi, Y. Lemoigne, E. Heidarian\",\"doi\":\"10.22038/NMJ.2019.06.000010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53), p53 gene expression (3.6 folds), reduction of cell invasion (9.82), and plating efficiency (5), thereby leading to the significant reduction of cell viability (40±0.9) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells. Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression.\",\"PeriodicalId\":18933,\"journal\":{\"name\":\"Nanomedicine Journal\",\"volume\":\"6 1\",\"pages\":\"232-240\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2019-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomedicine Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/NMJ.2019.06.000010\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NANOSCIENCE & NANOTECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/NMJ.2019.06.000010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 5

摘要

目的:纳米颗粒对癌症治疗的重要贡献已引起治疗关注。本研究旨在评估5-氟尿嘧啶(5-FU)和氧化锌纳米颗粒(ZnO-NP)作为多模式药物递送对人类乳腺癌症MCF-7细胞的协同作用。材料与方法:本研究采用MTT、克隆形成试验、流式细胞术、实时定量聚合酶链反应和蛋白质印迹等技术,评价5-FU和ZnO NPs单体或联合形式对MCF-7乳腺癌症细胞系细胞活力、集落形成、凋亡、p53基因表达和Bcl-2信号蛋白的影响。结果:在本研究中,5-FU与ZnO NPs在48小时内对MCF-7表现出协同作用。此外,与未处理的对照细胞相比,分别浓度为1µM和45µg/ml的5-FU和ZnO NP的组合表现出显著的细胞凋亡(79.53)、p53基因表达(3.6倍)、细胞侵袭减少(9.82)和铺板效率(5),从而导致细胞存活力显著降低(40±0.9)和Bcl-2抗凋亡蛋白降低。结论:ZnO NPs和5-FU联合应用对MCF-7人乳腺癌症细胞的协同作用是通过抑制Bcl-2和上调p53表达来实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity
Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53), p53 gene expression (3.6 folds), reduction of cell invasion (9.82), and plating efficiency (5), thereby leading to the significant reduction of cell viability (40±0.9) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells. Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信