用线性二次模型的理论计算和实验研究证明了高剂量电网技术中的旁观者反应

Q4 Health Professions
F. Pakniyat, S. Gholami, H. Nedaie, H. Mozdarani, A. Mahmoudzadeh, M. Salimi
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引用次数: 1

摘要

背景:线性二次型(LQ)方程作为放射治疗中最常见的公式,在模拟高剂量效应方面的准确性存在争议。本研究的目的是在理论计算和实验研究的基础上,证明SCC细胞系中网格处理的旁观者反应。材料和方法:采用线性二次模型计算最大剂量为10Gy的栅格场的等效均匀剂量。根据EUD的定义,期望从Grid和openfield单级分中获得相同的肿瘤存活率(SF)。在观察到差异后,通过培养基转移策略,从10Gy照射的供体到1.5Gy照射的受体,进行克隆发生和细胞凋亡测定,以研究旁观者的反应。结果:EUD等于4Gy,4Gy EUD和10Gy栅格场的SF分别为0.1±0.02和0.051±0.008。这些发现与他们生存平等的理论预期相矛盾。此外,旁观者克隆性细胞的死亡增加了约2.91倍(具有统计学意义);强调旁观者的反应作用。细胞凋亡结果表明,旁观者细胞经历了大约10%的增加,并且细胞凋亡率证实了在EUD中比在网格场中更少的克隆形成存活结果。结论:由于网格场的SF小于EUD,它显示了网格治疗的优势加上旁观者反应的表现;这在LQ方程中被忽略了,并且可能无法通过调制场的纯粹理论计算来证明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Demonstration of bystander response in high dose technique of grid using theoretical calculation by linear quadratic model along with experimental investigations
Background: The Linear Quadratic (LQ) equation as the most common formula in radiotherapy has a debatable accuracy in modeling high-dose effects. The purpose of this study was to demonstrate bystander response of the Grid treatment in SCC cell line, based on both theoretical calculations and experimental investigations. Materials and methods: The linear quadratic model was used to calculate the equivalent uniform dose (EUD) of a Grid-field with the 10 Gy maximum doses. According to the EUD definition, the identical tumor survival fraction (SF) was expected to obtain from both Grid and openfield single fraction. After observing the difference, the clonogenic and apoptosis assays were exerted to investigate bystander response via medium transfer strategy which was performed from 10Gy-irradiated donors to 1.5Gyirradiated recipients. Results: The EUD was equal to 4 Gy and the SF of 4 Gy EUD and 10 Gy Grid-field were 0.1 ± 0.02 and 0.051 ± 0.008, respectively. These findings contradicted the theoretical expectations of their survivals equality. Moreover, the bystander clonogenic cells death enhanced approximately by 2.91 times (statistically significant); highlighting the bystander response role. The apoptotic findings illustrated that the bystander cells experienced an approximately 10% increase and the apoptotic rate confirmed the clonogenic survival result which was less in the EUD than the Grid-field. Conclusion: Since the SF of the Grid-field was less than the EUD, it revealed the Grid therapeutic advantages plus bystander response manifestation; that was ignored in the LQ equation and may not be demonstrated by sheer theoretical calculations of the modulated-field.
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来源期刊
Iranian Journal of Radiation Research
Iranian Journal of Radiation Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
0.67
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Iranian Journal of Radiation Research (IJRR) publishes original scientific research and clinical investigations related to radiation oncology, radiation biology, and Medical and health physics. The clinical studies submitted for publication include experimental studies of combined modality treatment, especially chemoradiotherapy approaches, and relevant innovations in hyperthermia, brachytherapy, high LET irradiation, nuclear medicine, dosimetry, tumor imaging, radiation treatment planning, radiosensitizers, and radioprotectors. All manuscripts must pass stringent peer-review and only papers that are rated of high scientific quality are accepted.
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