慢性乙型肝炎病毒感染、肝硬化和肝癌患者外周血单个核细胞中乙型肝炎病毒基因组的检测和定量

IF 0.3 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Hepatitis Monthly Pub Date : 2022-01-09 DOI:10.5812/hepatmon.120982

V. Poortahmasebi, S. Alavian, A. Ghaziasadi, Arezou Azadi, M. Nasiritoosi, S. Jazayeri

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引用次数: 0

摘要

背景：多项研究表明，乙型肝炎病毒（HBV）存在于外周血单个核细胞（PBMC）中。目前尚不清楚HBV DNA和共价闭合环状DNA（cccDNA）是否能在不同阶段HBV感染患者的PBMC中出现。目的：本研究旨在比较慢性乙型肝炎（CHB）、肝硬化和肝细胞癌（HCC）患者PBMC中HBV DNA的检测以及cccDNA的定量和存在。方法：本研究对来自伊朗德黑兰的120名参与者（30名慢性乙型肝炎患者、30名肝硬化患者、30例HCC患者和30名健康对照）进行。采用酶联免疫吸附试验（ELISA）检测HBV血清学标志物。测定所有个体的PBMC的HBV DNA检测、定量和cccDNA的存在。结果：90例HBV患者中，58例（64.4%）PBMC中HBV DNA阳性。在13/30 CHB、20/30肝硬化和25/30 HCC患者的PBMC中检测到HBV DNA。此外，6例（20%）慢性乙型肝炎、13例（43.3%）肝硬化和16例（15.3%）HCC患者cccDNA阳性。血清HBV载量明显高于PBMC（P＜0.001），血清HBV DNA载量与患者PBMC呈正相关。此外，血清和PBMC的HBV DNA量与乙型肝炎e抗原（HBeAg）状态有显著相关性。结论：PBMC中HBV含量与血清HBV载量相关。PBMC中的HBV基因组可能是HBV疾病进展的危险因素。

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Detection and Quantification of Hepatitis B Virus Genomes in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Virus Infection, Cirrhosis, and Hepatocellular Carcinoma Patients

Background: Several studies have revealed that the hepatitis B virus (HBV) exists in peripheral blood mononuclear cells (PBMCs). It remains poorly understood whether HBV DNA and covalently closed circular DNA (cccDNA) can emerge in PBMCs of patients with different stages of HBV infection. Objectives: This study aimed to compare the detection of HBV DNA and quantification and presence of cccDNA within PBMC from patients with chronic hepatitis B (CHB), cirrhosis, and hepatocellular carcinoma (HCC). Methods: The present study was conducted on 120 participants (30 CHB patients, 30 cirrhosis patients, 30 HCC patients, and 30 healthy controls) from Tehran, Iran. HBV serological markers were tested by enzyme-linked immunosorbent assay (ELISA). PBMCs of all individuals were assayed for HBV DNA detection, quantification, and the presence of cccDNA. Results: Of 90 HBV patients, 58 (64.4%) were positive for HBV DNA in PBMCs. HBV DNA was detected in PBMCs isolated from 13/30 CHB, 20/30 cirrhosis, and 25/30 HCC patients. In addition, 6 (20%) CHB, 13 (43.3%) cirrhosis, and 16 (15.3%) HCC patients were cccDNA positive. The HBV viral loads in serums were statistically higher than the HBV viral loads of PBMCs (P < 0.001). A positive correlation was found between HBV DNA loads in serums and PBMCs of patients. Moreover, HBV DNA quantity of serums and PBMCs showed a significant association in terms of hepatitis B e antigen (HBeAg) status. Conclusions: HBV quantity in PBMCs correlated with serum HBV viral loads. HBV genomes in PBMCs may be a risk factor for HBV disease progression.

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来源期刊

Hepatitis Monthly 医学-胃肠肝病学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Hepatitis Monthly is a clinical journal which is informative to all practitioners like gastroenterologists, hepatologists and infectious disease specialists and internists. This authoritative clinical journal was founded by Professor Seyed-Moayed Alavian in 2002. The Journal context is devoted to the particular compilation of the latest worldwide and interdisciplinary approach and findings including original manuscripts, meta-analyses and reviews, health economic papers, debates and consensus statements of the clinical relevance of hepatological field especially liver diseases. In addition, consensus evidential reports not only highlight the new observations, original research, and results accompanied by innovative treatments and all the other relevant topics but also include highlighting disease mechanisms or important clinical observations and letters on articles published in the journal.