两种医学上重要的蝎子的毒液成分特征,Hottentota tamulus(Fabricius,1798)和Androctonus finitimus(Pocock,1897)

IF 0.2 4区 农林科学 Q4 ENTOMOLOGY
H. M. Tahir, Saadia Tobassum, Shaukat Ali, Muhammad Tariq Zahid, M. Arshad, Qurratulann Afza Gardner, M. Ahsan
{"title":"两种医学上重要的蝎子的毒液成分特征,Hottentota tamulus(Fabricius,1798)和Androctonus finitimus(Pocock,1897)","authors":"H. M. Tahir, Saadia Tobassum, Shaukat Ali, Muhammad Tariq Zahid, M. Arshad, Qurratulann Afza Gardner, M. Ahsan","doi":"10.2317/0022-8567-93.3.230","DOIUrl":null,"url":null,"abstract":"ABSTRACT: Scorpion venom is being used for many biomedical applications. In the present study, venom components of Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897) were characterized. Venom was extracted using the electrical stimulation method and subjected to High Performance Liquid Chromatography (HPLC) for characterization of its components from which dominant fractions were collected and dried in a concentrator. Crude venom and selected fractions were further analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to obtain mass fingerprint data. Over 100 components ranging from 3,000 – 8,000 m/z in molecular masses were detected in the venom of both scorpion species. Some of them were analogous to a short chain and long chain toxins, which act on potassium and sodium ion channels, respectively. The venom components may be used for the development of novel drugs, e.g. drugs for neurological disorders, cardiovascular diseases, analgesics and cancer.","PeriodicalId":17396,"journal":{"name":"Journal of the Kansas Entomological Society","volume":"93 1","pages":"230 - 245"},"PeriodicalIF":0.2000,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Characterization of Venom Components of Two Medically Important Scorpion Species, Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897)\",\"authors\":\"H. M. Tahir, Saadia Tobassum, Shaukat Ali, Muhammad Tariq Zahid, M. Arshad, Qurratulann Afza Gardner, M. Ahsan\",\"doi\":\"10.2317/0022-8567-93.3.230\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT: Scorpion venom is being used for many biomedical applications. In the present study, venom components of Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897) were characterized. Venom was extracted using the electrical stimulation method and subjected to High Performance Liquid Chromatography (HPLC) for characterization of its components from which dominant fractions were collected and dried in a concentrator. Crude venom and selected fractions were further analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to obtain mass fingerprint data. Over 100 components ranging from 3,000 – 8,000 m/z in molecular masses were detected in the venom of both scorpion species. Some of them were analogous to a short chain and long chain toxins, which act on potassium and sodium ion channels, respectively. The venom components may be used for the development of novel drugs, e.g. drugs for neurological disorders, cardiovascular diseases, analgesics and cancer.\",\"PeriodicalId\":17396,\"journal\":{\"name\":\"Journal of the Kansas Entomological Society\",\"volume\":\"93 1\",\"pages\":\"230 - 245\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2021-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Kansas Entomological Society\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2317/0022-8567-93.3.230\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Kansas Entomological Society","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2317/0022-8567-93.3.230","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 1

摘要

摘要:蝎毒被广泛应用于生物医学领域。在本研究中,对霍滕托塔(Fabricius,1798)和安德克通(Androctonus finitimus,Pocock,1897)的毒液成分进行了表征。使用电刺激法提取毒液,并进行高效液相色谱(HPLC)以表征其成分,从中收集主要组分并在浓缩器中干燥。通过基质辅助激光解吸/电离飞行时间质谱法(MALDI-TOF MS)对粗毒液和所选组分进行进一步分析,以获得质量指纹数据。在这两种蝎子的毒液中都检测到了100多种分子量在3000–8000 m/z之间的成分。其中一些类似于短链和长链毒素,分别作用于钾离子通道和钠离子通道。毒素组分可用于开发新的药物,例如用于神经疾病、心血管疾病、止痛药和癌症的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Venom Components of Two Medically Important Scorpion Species, Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897)
ABSTRACT: Scorpion venom is being used for many biomedical applications. In the present study, venom components of Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897) were characterized. Venom was extracted using the electrical stimulation method and subjected to High Performance Liquid Chromatography (HPLC) for characterization of its components from which dominant fractions were collected and dried in a concentrator. Crude venom and selected fractions were further analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to obtain mass fingerprint data. Over 100 components ranging from 3,000 – 8,000 m/z in molecular masses were detected in the venom of both scorpion species. Some of them were analogous to a short chain and long chain toxins, which act on potassium and sodium ion channels, respectively. The venom components may be used for the development of novel drugs, e.g. drugs for neurological disorders, cardiovascular diseases, analgesics and cancer.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.60
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信