{"title":"橡胶植物棒孢菌落叶病抗性QTL的初步检测","authors":"F. Oktavia, S. Sudarsono, D. Dinarti","doi":"10.13057/NUSBIOSCI/N130108","DOIUrl":null,"url":null,"abstract":"Abstract. Oktavia F, Sudarsono, Dinarti D. 2021. Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant. Nusantara Bioscience 13: 53-61. Corynespora Leaf Fall (CLF) disease caused by Corynespora cassiicola fungus is one of the most important diseases of rubber trees. Conventional breeding to obtain the resistant rubber clones was constrained by the long time required for selection processes. This study was aimed to identify resistant loci linked with CLF disease on the rubber tree. Analysis was conducted on 104 F1 of BPM 1 x RRIM 600. The resistance evaluation of the population had been done against two C. cassiicola isolates and the molecular analysis was generated by using 28 selected SSR markers. There was a phenotypic variation in F1 resistance to both isolates with leaf wilting intensities ranged from 5.2% to 33.4% on CC-06 isolate, and from 3.5% to 36.4% on CC-22 isolate. There was no QTL identified on the genetic linkage map, but a single marker analysis showed that some loci associated with CLF disease. The associated loci can be used as a preliminary information to develop molecular markers linked with resistance to CLF disease to assist the rubber breeding programs.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":"1 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant\",\"authors\":\"F. Oktavia, S. Sudarsono, D. Dinarti\",\"doi\":\"10.13057/NUSBIOSCI/N130108\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract. Oktavia F, Sudarsono, Dinarti D. 2021. Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant. Nusantara Bioscience 13: 53-61. Corynespora Leaf Fall (CLF) disease caused by Corynespora cassiicola fungus is one of the most important diseases of rubber trees. Conventional breeding to obtain the resistant rubber clones was constrained by the long time required for selection processes. This study was aimed to identify resistant loci linked with CLF disease on the rubber tree. Analysis was conducted on 104 F1 of BPM 1 x RRIM 600. The resistance evaluation of the population had been done against two C. cassiicola isolates and the molecular analysis was generated by using 28 selected SSR markers. There was a phenotypic variation in F1 resistance to both isolates with leaf wilting intensities ranged from 5.2% to 33.4% on CC-06 isolate, and from 3.5% to 36.4% on CC-22 isolate. There was no QTL identified on the genetic linkage map, but a single marker analysis showed that some loci associated with CLF disease. The associated loci can be used as a preliminary information to develop molecular markers linked with resistance to CLF disease to assist the rubber breeding programs.\",\"PeriodicalId\":19481,\"journal\":{\"name\":\"Nusantara Bioscience\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2021-02-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nusantara Bioscience\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.13057/NUSBIOSCI/N130108\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nusantara Bioscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.13057/NUSBIOSCI/N130108","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 2
摘要
摘要Oktavia F，Sudarsono，Dinarti D.2021。橡胶植物棒孢菌落叶病抗性QTL的初步检测。Nusantara Bioscience 13:53-61。由黑胶棒孢菌引起的落叶棒孢病是橡胶树最重要的病害之一。获得抗性橡胶无性系的常规育种受到选择过程所需的长时间的限制。本研究旨在鉴定橡胶树上与CLF病相关的抗性基因座。对BPM 1 x RRIM 600的104 F1进行分析。对该群体对两个决明子菌株进行了抗性评价，并利用28个选择的SSR标记进行了分子分析。F1对两个分离株的抗性存在表型变异，CC-06分离株的叶片萎蔫强度为5.2%-33.4%，CC-22分离株的叶萎蔫程度为3.5%-36.4%。遗传连锁图上没有鉴定出QTL，但单标记分析表明，一些位点与CLF疾病有关。相关基因座可作为开发与CLF疾病抗性相关的分子标记的初步信息，以帮助橡胶育种计划。
Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant
Abstract. Oktavia F, Sudarsono, Dinarti D. 2021. Preliminary QTL detection for Corynespora Leaf Fall disease resistance in rubber plant. Nusantara Bioscience 13: 53-61. Corynespora Leaf Fall (CLF) disease caused by Corynespora cassiicola fungus is one of the most important diseases of rubber trees. Conventional breeding to obtain the resistant rubber clones was constrained by the long time required for selection processes. This study was aimed to identify resistant loci linked with CLF disease on the rubber tree. Analysis was conducted on 104 F1 of BPM 1 x RRIM 600. The resistance evaluation of the population had been done against two C. cassiicola isolates and the molecular analysis was generated by using 28 selected SSR markers. There was a phenotypic variation in F1 resistance to both isolates with leaf wilting intensities ranged from 5.2% to 33.4% on CC-06 isolate, and from 3.5% to 36.4% on CC-22 isolate. There was no QTL identified on the genetic linkage map, but a single marker analysis showed that some loci associated with CLF disease. The associated loci can be used as a preliminary information to develop molecular markers linked with resistance to CLF disease to assist the rubber breeding programs.
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