{"title":"基于RNA干扰的赤谷三棱红粉甲虫免疫信号通路中Caspar、DREDD和FADD基因的表征(鞘翅目:Tenebrionidae)","authors":"K. Yokoi, Wataru Ito, Daiki Kato, K. Miura","doi":"10.14411/eje.2022.003","DOIUrl":null,"url":null,"abstract":"We previously demonstrated that two immune signaling pathways, Toll and IMD, were concomitantly activated in the model beetle Tribolium castaneum by challenges to their immune system by several species of microbes, including Gram-positive and -negative bacteria as well as yeast. This contrasts with the Drosophila immune system in which more specifi c pathway activation depending on the type of microbe is well established. We suggest that the activation of an indiscriminate immune pathway in T. castaneum is due in part to an unselective recognition of pathogen-associated molecular patterns by the extracellular sensing modules of the two pathways. In order to obtain a more detailed understanding of the T. castaneum immune pathway, we investigated whether potential components of the T. castaneum IMD pathway, Caspar, DREDD and FADD, are involved in immune reactions triggered by microbial challenges. A sequence analysis of these three genes with the orthologues of other species, including insects, mouse and human, indicated that T. castaneum Caspar, DREDD and FADD functioned as immune signal transducers, which are usually induced by microbial challenges. However, these genes were not induced by microbial challenges. To establish whether these genes are involved in immune reactions, we used RNA interference-mediated knockdown of these genes to assess the microbial induction levels of the representative read-out antimicrobial peptide genes of the respective classes. The results indicated that these genes encode the canonical constituents of the IMD pathway of this beetle. DREDD and FADD infl uenced the induction of Toll-dependent antimicrobial peptide genes, providing novel crosstalk points between the two immune pathways, which appears to support indiscriminate pathway activation in T. castaneum. Furthermore, the phenotypes of DREDD or FADD knockdown pupae challenged by the two model bacterial pathogens correlated with AMP gene induction in the respective knockdowns, indicating that these intracellular factors contributed to antibacterial host defenses. * These authors equally contributed. INTRODUCTION The insect immune system is solely composed of innate immune reactions that utilize germ line-encoded receptors to detect the invasion of several distinct pathogens (Hultmark, 2003; Ferrandon et al., 2007; Lemaitre & Hoffmann, 2007). When recognized, invading pathogens are attacked by a battery of innate immune responses. Insect immune responses as well as vertebrate immune responses may be conveniently divided into cellular and humoral immunities, the latter of which involves the systemic and robust production of antimicrobial peptides (AMPs) as a major and well-characterized response. Intensive studies on the model organism Drosophila melanogaster (Meigen) over the past two decades have delineated the paths and facEur. J. Entomol. 119: 23–35, 2022 doi: 10.14411/eje.2022.003","PeriodicalId":11940,"journal":{"name":"European Journal of Entomology","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"RNA interference-based characterization of Caspar, DREDD and FADD genes in immune signaling pathways of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)\",\"authors\":\"K. Yokoi, Wataru Ito, Daiki Kato, K. Miura\",\"doi\":\"10.14411/eje.2022.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We previously demonstrated that two immune signaling pathways, Toll and IMD, were concomitantly activated in the model beetle Tribolium castaneum by challenges to their immune system by several species of microbes, including Gram-positive and -negative bacteria as well as yeast. This contrasts with the Drosophila immune system in which more specifi c pathway activation depending on the type of microbe is well established. We suggest that the activation of an indiscriminate immune pathway in T. castaneum is due in part to an unselective recognition of pathogen-associated molecular patterns by the extracellular sensing modules of the two pathways. In order to obtain a more detailed understanding of the T. castaneum immune pathway, we investigated whether potential components of the T. castaneum IMD pathway, Caspar, DREDD and FADD, are involved in immune reactions triggered by microbial challenges. A sequence analysis of these three genes with the orthologues of other species, including insects, mouse and human, indicated that T. castaneum Caspar, DREDD and FADD functioned as immune signal transducers, which are usually induced by microbial challenges. However, these genes were not induced by microbial challenges. To establish whether these genes are involved in immune reactions, we used RNA interference-mediated knockdown of these genes to assess the microbial induction levels of the representative read-out antimicrobial peptide genes of the respective classes. The results indicated that these genes encode the canonical constituents of the IMD pathway of this beetle. DREDD and FADD infl uenced the induction of Toll-dependent antimicrobial peptide genes, providing novel crosstalk points between the two immune pathways, which appears to support indiscriminate pathway activation in T. castaneum. Furthermore, the phenotypes of DREDD or FADD knockdown pupae challenged by the two model bacterial pathogens correlated with AMP gene induction in the respective knockdowns, indicating that these intracellular factors contributed to antibacterial host defenses. * These authors equally contributed. INTRODUCTION The insect immune system is solely composed of innate immune reactions that utilize germ line-encoded receptors to detect the invasion of several distinct pathogens (Hultmark, 2003; Ferrandon et al., 2007; Lemaitre & Hoffmann, 2007). When recognized, invading pathogens are attacked by a battery of innate immune responses. Insect immune responses as well as vertebrate immune responses may be conveniently divided into cellular and humoral immunities, the latter of which involves the systemic and robust production of antimicrobial peptides (AMPs) as a major and well-characterized response. Intensive studies on the model organism Drosophila melanogaster (Meigen) over the past two decades have delineated the paths and facEur. J. 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RNA interference-based characterization of Caspar, DREDD and FADD genes in immune signaling pathways of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
We previously demonstrated that two immune signaling pathways, Toll and IMD, were concomitantly activated in the model beetle Tribolium castaneum by challenges to their immune system by several species of microbes, including Gram-positive and -negative bacteria as well as yeast. This contrasts with the Drosophila immune system in which more specifi c pathway activation depending on the type of microbe is well established. We suggest that the activation of an indiscriminate immune pathway in T. castaneum is due in part to an unselective recognition of pathogen-associated molecular patterns by the extracellular sensing modules of the two pathways. In order to obtain a more detailed understanding of the T. castaneum immune pathway, we investigated whether potential components of the T. castaneum IMD pathway, Caspar, DREDD and FADD, are involved in immune reactions triggered by microbial challenges. A sequence analysis of these three genes with the orthologues of other species, including insects, mouse and human, indicated that T. castaneum Caspar, DREDD and FADD functioned as immune signal transducers, which are usually induced by microbial challenges. However, these genes were not induced by microbial challenges. To establish whether these genes are involved in immune reactions, we used RNA interference-mediated knockdown of these genes to assess the microbial induction levels of the representative read-out antimicrobial peptide genes of the respective classes. The results indicated that these genes encode the canonical constituents of the IMD pathway of this beetle. DREDD and FADD infl uenced the induction of Toll-dependent antimicrobial peptide genes, providing novel crosstalk points between the two immune pathways, which appears to support indiscriminate pathway activation in T. castaneum. Furthermore, the phenotypes of DREDD or FADD knockdown pupae challenged by the two model bacterial pathogens correlated with AMP gene induction in the respective knockdowns, indicating that these intracellular factors contributed to antibacterial host defenses. * These authors equally contributed. INTRODUCTION The insect immune system is solely composed of innate immune reactions that utilize germ line-encoded receptors to detect the invasion of several distinct pathogens (Hultmark, 2003; Ferrandon et al., 2007; Lemaitre & Hoffmann, 2007). When recognized, invading pathogens are attacked by a battery of innate immune responses. Insect immune responses as well as vertebrate immune responses may be conveniently divided into cellular and humoral immunities, the latter of which involves the systemic and robust production of antimicrobial peptides (AMPs) as a major and well-characterized response. Intensive studies on the model organism Drosophila melanogaster (Meigen) over the past two decades have delineated the paths and facEur. J. Entomol. 119: 23–35, 2022 doi: 10.14411/eje.2022.003
期刊介绍:
EJE publishes original articles, reviews and points of view on all aspects of entomology. There are no restrictions on geographic region or taxon (Myriapoda, Chelicerata and terrestrial Crustacea included). Comprehensive studies and comparative/experimental approaches are preferred and the following types of manuscripts will usually be declined:
- Descriptive alpha-taxonomic studies unless the paper is markedly comprehensive/revisional taxonomically or regionally, and/or significantly improves our knowledge of comparative morphology, relationships or biogeography of the higher taxon concerned;
- Other purely or predominantly descriptive or enumerative papers [such as (ultra)structural and functional details, life tables, host records, distributional records and faunistic surveys, compiled checklists, etc.] unless they are exceptionally comprehensive or concern data or taxa of particular entomological (e.g., phylogenetic) interest;
- Papers evaluating the effect of chemicals (including pesticides, plant extracts, attractants or repellents, etc.), irradiation, pathogens, or dealing with other data of predominantly agro-economic impact without general entomological relevance.