Jean Poll Alva-Araujo, Gustavo A. Cano-Arcos, Juan G. Juscamaita-Morales, Lawrence Quipuzco Ushñahua
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引用次数: 0
摘要
本研究的目的是评估乳酸菌(LAB)去除生活废水中存在的病原体的能力,这些废水是在生态旅游线路Lomas de Lucumo(秘鲁利马)产生的。pH值的降低被用作去除处理水中的粪便大肠菌群的指标。实验包括36个处理,其由糖蜜和乳酸菌接种物(B-Lac)的不同混合物组成,比例为0、1、3、5、7和10%(v/v),废水的固定比例为200ml,采用完全随机设计(CRD),因子排列为6x6。第三天的pH值使用方差分析(ANOVA)进行评估,然后进行Tukey的平均差异范围检验(p<0.05)。在第一阶段对不同的处理进行9天的分析,然后选择最好的三种进行第二次评估:T16(3%糖蜜和5%B-Lac)、T22(5%糖蜜和5%B-Lac)和T33(10%糖蜜和3%B-Lac;结果表明,两个变量之间存在显著的交互作用。最后,处理T16被选为最有效的处理,在短时间(3天)内达到4.08的pH值,确保完全去除废水中的粪便大肠菌群(9.65x10 5 MPN/100 ml)。
Reduction of pH by homolactic fermentation as indicator of fecal coliform inactivation in wastewater
The purpose of this research was to evaluate the capability of lactic acid bacteria (LAB) to remove pathogens present in domestic wastewater, generated in the eco-touristic circuit Lomas de Lucumo (Lima, Peru). The pH decrease was used as an indicator of the elimination of fecal coliform bacteria in the treated water. Experiments included 36 treatments, which consisted of different mixtures of sugar molasses and a lactic acid bacteria inoculum (B-Lac) in proportions of 0, 1, 3, 5, 7 and 10% (v/v) and wastewater in a fixed proportion of 200 ml, under a completely randomized design (CRD) with factorial arrangement 6x6. The pH values on the third day were evaluated using an Analysis of Variance (ANOVA) followed by Tukey´s range test for mean differences (p < 0.05). The different treatments were analyzed in a first stage for 9 days, after which the best three were selected for a second evaluation: T16 (3% molasses and 5% B-Lac), T22 (5% molasses and 5% B-Lac) and T33 (10% molasses and 3% B-Lac); results show that the interaction effect between the two variables is significant. Finally, treatment T16 was selected as the most efficient, reaching a pH of 4.08 in a short time (3 days) that assured the complete removal of fecal coliform bacteria (9.65x10 5 MPN/100 ml) in the wastewater.