与自然感染相比，接种疫苗个体的SARS-CoV-2刺突受体结合域的定量研究

Open Access Macedonian Journal of Medical Sciences Pub Date : 2023-04-14 DOI:10.3889/oamjms.2023.11611

Maria Yasintha Lihawa, E. A. Datau, E. Surachmanto, J. Soekarno, Budi Tulaka

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引用次数: 0

摘要

背景：2019冠状病毒病（新冠肺炎）大流行已经持续了2年多，有各种治疗和诊断方法。严重急性呼吸综合征冠状病毒2型刺突蛋白的受体结合域（RBD）是最珍贵的结构之一，长期以来，人们对其功能进行了深入研究，并成为包括疫苗在内的各种诊断方法和治疗的靶点。刺突RBD（sRBD）抗体计数可能是接种疫苗和感染个体抗体反应的参数。然而，没有进行直接比较。目的：本研究旨在比较自然感染者和接种疫苗者的sRBD抗体计数。方法：我们对49名感染患者进行了横断面研究，接种疫苗的人包括在这项研究中，他们来自Manado的R.D.Kandou教授博士医院。参与者接受了新冠肺炎抗体测试，使用增强的“化学发光”免疫测定法定量分析抗sRBD IgG。然后使用IBM社会科学统计软件包25.0版和Mann-Whitney非参数检验对结果进行分析和比较。结果：该研究显示，与接种疫苗的组相比，自然感染组的中位抗体计数更高（132.70对11.95 U/mL；p<0.001）。应进一步研究该主题，以确定更大范围的比较。结论：自然感染者的s-RBD抗体滴度明显高于接种者。

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Quantitative SARS-CoV-2 Spike Receptor-Binding Domain on Vaccinated Individuals Compared to Natural Infection

BACKGROUND: Coronavirus disease 2019 (COVID-19) pandemic has been going on for more than 2 years, with various treatments and diagnostic methods available. One of the most prized structures, the receptor-binding domain (RBD) of the spike protein in severe acute respiratory syndrome coronavirus 2 has long been thoroughly researched for its function and becoming the target for various diagnostic methods and treatments, including a vaccine. The spike-RBD (sRBD) antibody count might be the parameter for antibody response in vaccinated and infected individuals. However, no direct comparison is made. AIM: The study aims to compare the sRBD antibody count in the naturally infected individuals to the vaccinated ones. METHODS: We conducted a cross-sectional study with 49 participants of the infected patients, and vaccinated individuals were included in this study from Prof. Dr. R. D. Kandou Hospital, Manado. The participants underwent a COVID-19 antibody test, using enhanced “Chemiluminescence” Immuno assay to analyze the anti-sRBD IgG quantitatively. Results were then analyzed and compared using IBM Statistical Package for Social Sciences ver 25.0 with Mann−Whitney non-parametric test. RESULTS: The study shows a higher median antibody count in the naturally infected group compared to the vaccinated group (132.70 vs. 11.95 U/mL; p < 0.001). Further studies on the topic should be conducted to determine the comparison on a larger scale. CONCLUSION: The s-RBD antibody titer is significantly higher in naturally infected patients than in vaccinated individuals.

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来源期刊

Open Access Macedonian Journal of Medical Sciences Medicine-Medicine (all)

自引率

0.00%

发文量

1225

期刊介绍： Open Access Macedonian Journal of Medical Sciences (OAMJMS) [formerly known as Macedonian Journal of Medical Sciences] is a top-tier open access medical science journal published by the ID Design 2012/DOOEL Skopje, Rajko Zhinzifov No 48, 1000 Skopje, Republic of Macedonia. OAMJMS is an international, modern, general medical journal covering all areas in the medical sciences, from basic studies to large clinical trials and cost-effectiveness analyses. We publish mostly human studies that substantially enhance our understanding of disease epidemiology, etiology, and physiology; the development of prognostic and diagnostic technologies; trials that test the efficacy of specific interventions and those that compare different treatments; and systematic reviews. We aim to promote translation of basic research into clinical investigation, and of clinical evidence into practice. We publish occasional studies in animal models when they report outstanding research findings that are highly clinically relevant. Our audience is the international medical community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world. OAMJMS is published quarterly online version. The Open Access Macedonian Journal of Medical Sciences (OAMJMS) publishes Medical Informatics, Basic Science, Clinical Science, Case Report, Brief Communication, Public Health, Public Policy, and Review Article from all fields of medicine and related fields. This journal also publishes, continuously or occasionally, the bibliographies of the members of the Society, medical history, medical publications, thesis abstracts, book reviews, reports on meetings, information on future meetings, important events and dates, and various headings which contribute to the development of the corresponding scientific field.