阿月浑子对溶血性贫血大鼠肾功能的保护作用

IF 1.1 Q4 PHARMACOLOGY & PHARMACY
Najmeh Parvaz, Fatemeh Amin, N. Askari, Morteza Khademalhosseini, S. K. Falahatipour, S. Falahati-pour
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引用次数: 3

摘要

背景和目的:黄连木被认为是具有抗氧化和肾保护活性等治疗功能的独特物质来源。本研究旨在探讨黄连木果皮水提物对苯肼致贫血模型大鼠肾脏的生化和组织病理学影响。方法:采用浸渍法提取胡杨果皮。在动物实验中,将大鼠分为六组,在没有或存在苯肼提取物的情况下暴露于苯肼中两天。采用生化和组织病理学检测肾脏变化。在代谢笼中收集尿液样本,测定总尿量、肌酐和24小时蛋白尿(蛋白/肌酐比值)。采用ELISA法检测血清过氧化氢酶、丙二醛和超氧化物歧化酶作为氧化应激标志物。结果:与对照组相比,苯肼引起的肾损伤表现为尿、血清尿素、肌酐水平、钠离子和钾离子的显著变化;然而,提取物治疗明显减轻肾损伤。80 mg/kg提取物可显著降低贫血动物的肌酐和蛋白尿(p<0.05), 160 mg/kg提取物可使贫血动物的蛋白质和肌酐降至正常水平。结论:24小时蛋白和肌酐可作为贫血肾损伤的标志物,定期测定可用于发现贫血肾问题的危险性。这些结果表明,给药可能通过减轻肾脏炎症来减轻肾脏损伤和功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Protective Effect of Pistacia vera Pericarp on Kidney Function in Rats with Hemolytic Anemia
Background and objectives: Pistacia vera is known as a source of unique materials with therapeutic function such as antioxidant and nephron-protective activities. This study aimed to identify the biochemical and histopathological effects of Pistacia vera pericarp aqueous extract on the kidney in phenylhydrazine-induced anemia model in rats. Methods: Extraction of the P. vera pericarp was carried out by maceration technique. For animal study, the rats were studied in six groups and were exposed to phenylhydrazine for two days in the absence or presence of the extract. Renal changes were measured using biochemical and histopathological assays. The urine samples were collected in metabolic cages for total urine volume, creatinine, and 24-hour proteinuria measurement with the protein/creatinine ratio. Serum catalase, malondialdehyde and superoxide dismutase as oxidative stress markers were examined using ELISA test. Results: Phenylhydrazine induced kidney injuries evidenced by significant changes of urine, serum urea, creatinine levels, sodium, and potassium ions in comparison to the control group; however, the extract treatment significantly decreased kidney injuries. Administration of 80 mg/kg of the extract significantly reduced the creatinine and proteinuria in treated animals (p<0.05) but 160 mg/kg of extract helped the anemic animals to reduce protein and creatinine to normal levels. Conclusion: Twenty-four hours protein and creatinine can be used as markers of renal injuries in anemia and their regular measurement can be useful to find the risk of renal problems in anemia. These results revealed that P. vera pericarp administration may decrease renal injuries and dysfunction by reducing inflammation in the kidney.
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来源期刊
Research Journal of Pharmacognosy
Research Journal of Pharmacognosy PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
20.00%
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0
审稿时长
8 weeks
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