大麻泽兰对人乳腺癌MCF-7细胞株的细胞毒性作用

IF 1.1 Q4 PHARMACOLOGY & PHARMACY
Morteza Abuali, M. Ardekani, H. Rezadoost, M. Vazirian, Majid Balaei-Kahnamoei, M. Hamzeloo-Moghadam
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引用次数: 0

摘要

背景和目的:癌症是世界上第二大死亡原因。女性中最常见的癌症是癌症。在癌症治疗中使用植物衍生产品可以减少不良副作用。世界各地的广泛研究发现了可用于治疗某些类型癌症的草药化合物。根据先前的研究,大麻泽兰的甲醇提取物在一些癌症细胞系中显示出细胞毒性作用。本研究采用生物测定法对大麻素进行分级分离,评价其在MCF-7乳腺癌症细胞系中的细胞毒性。方法:采用浸渍法从药材中提取。采用柱色谱法对提取物进行分离纯化。通过MTT法评估了不同提取物对MCF-7细胞系的细胞毒性活性,并通过生物测定引导的分级分离分离出一种化合物。测定了分离的化合物的细胞毒性和凋亡特性。结果:氯仿提取物活性最高,IC50为21.39±3.24μg/mL,其次为正己烷和甲醇提取物,IC50分别为60.23±2.16μg/mL和81.74±3.41μg/mL。氯仿提取物中各亚组分(1-6)的IC50分别为60.83±2.56μg/mL、58.93±2.73μg/mL,37.5±3.65μg/mL和7.86±1.34μg/mL。采用生物测定法从三氯甲烷提取物中分离得到倍半萜内酯——大麻素内酯。IC50为13±2.45μ。在Annexin V/PI测定中,在13μg/mL浓度下,大麻素内酯诱导MCF-7细胞凋亡46.91%。结论:Eucannabinolideis是癌症药物发现研究的一个很有前途的候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line
Background and objectives: Cancer is known to be the second cause of death around the world. The most prevalent cancer among women is breast cancer. Use of plant-derived products in cancer treatment may reduce adverse side effects. Extensive research around the world has led to the discovery of herbal compounds that can be used to treat some types of cancer. According to previous studies, the methanol extract of Eupatorium cannabinum has shown cytotoxic effects in some cancer cell lines. In the present study, bioassay guided fractionation and isolation was conducted on E. cannabinum to evaluate the cytotoxic activityin MCF-7 breast cancer cell line. Methods: The Extraction from theaerial parts was performed by maceration method. Isolation and purification of the extracts were performed by column chromatography. The cytotoxic activity of different extracts of E. cannabinum was evaluated against MCF-7 cell line by MTT assay and a compound was isolated according to bioassay guided fractionation. The cytotoxic activity and apoptotic property of the isolated compound was determined. Results: The chloroform extract was the most active one with IC50 of 21.39±3.24 μg/mL followed by the n-hexane and methanol extracts with IC50values of 60.23±2.16 μg/mL and 81.74±3.41 μg/mL, respectively. IC50 of subfractions (1-6) from the chloroform extract were 60.83±2.56 μg/mL, 58.93±2.73 μg/mL, 37.5±3.65 μg/mL, 7.86±1.34 μg/mL, 10.61±2.34 μg/mL and 13.77±4.17 μg/mL, respectively. Eucannabinolide, a sesquiterpene lactone, was isolated from the chloroform extract according to bioassay guided fractionation. Its IC50 was found to be 13±2.45 μg/mL. Eucannabinolide induced 46.91% apoptosis at concentration of 13 μg/mL in MCF-7 cell line in Annexin V/PI assay. Conclusion: Eucannabinolideis a promising candidate for further breast cancer drug discovery studies.
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来源期刊
Research Journal of Pharmacognosy
Research Journal of Pharmacognosy PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
20.00%
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审稿时长
8 weeks
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