RFLP-PCR技术在伊拉克尼尼夫省根瘤菌遗传多样性和系统发育研究

IF 1.6 Q3 PLANT SCIENCES
M. A. Al-Shakarchi
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引用次数: 0

摘要

根瘤菌是重要的细菌,在大气固氮中起着至关重要的作用。此外,它还能提高土壤肥力,提高作物产量,保护土壤免受无机肥料使用造成的污染。从81个细菌样本中,从9种豆科作物中分离出的9个菌株在伊拉克尼尼微省的9个不同地点具有经济价值。分离株通过表型和培养特征以及宿主专业化测试进行初步诊断。对所选细菌分离物进行了分子表征。采用聚合酶链反应(PCR)技术,采用正向引物27F和反向引物1541R进行16S rRNA区域扩增。3种核酸内切酶的限制性片段长度多态性(RFLP-PCR)结果表明,EcoRI在各分离株中序列相似,但在MspI和HaeIII中差异显著。利用统计程序MVSP 3.22对结果进行分析,将分离物的相似度分为3组。结果显示,菌株之间存在遗传差异和抑制酶,这为识别菌株之间的相似性提供了更准确的结果,因为菌株分离的位置和环境条件存在差异,以及这些地区使用的不同农业工艺。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
GENETIC DIVERSITY AND PHYLOGENY OF RHIZOBIA ISOLATED FROM NODULES USING RFLP-PCR TECHNIQUE IN NINEVEH PROVINCE, IRAQ
Rhizobia are important bacteria, playing a vital role in atmospheric nitrogen fixation. In addition, it increases soil fertility, enhancing crop production and preserving the soil from pollution resulting from inorganic fertilizers’ use. From 81 bacteria samples, nine isolates segregated from nine types of leguminous crops are economically valuable for cultivation within nine different locations in Nineveh Governorate, Iraq. The isolates underwent initial diagnosis by phenotypical and cultural characteristics and host specialization tests. Molecular characterization also ran on the selected bacterial isolates. Performing the 16S rRNA region amplification applied forward primer 27F and a reverse primer 1541R using the polymerase chain reaction (PCR) technique. The results of restriction fragment length polymorphism (RFLP-PCR) employing three endonuclease enzymes showed that EcoRI had similar sequences among the isolates under study but significantly different for MspI and HaeIII. Based on the analysis of results using the statistical program MVSP version 3.22, the degree of similarity divided the isolates into three groups. Results revealed that there were genetic differences between the isolates and the suppressing enzymes, providing more accurate results in recognizing the similarity among the bacterial isolates as a result of the difference in the location and environmental conditions from which the bacteria were isolated, in addition to the different agricultural processes used for those regions.
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来源期刊
Sabrao Journal of Breeding and Genetics
Sabrao Journal of Breeding and Genetics 农林科学-奶制品与动物科学
CiteScore
1.90
自引率
50.00%
发文量
63
期刊介绍: The SABRAO Journal of Breeding and Genetics is an international journal of plant breeding and genetics research and was first published in 1969. It is the official publication of the Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO). Its objectives are to: promote the international exchange of research information on plant breeding and genetics, by describing new research findings, or ideas of a basic or practical nature; and be a medium for the exchange of ideas and news regarding members of the Society. The Journal gives priority to articles that are of direct relevance to plant breeders and with emphasis on the Asian region. Invited for publication are research articles, short communications, methods, reviews, commentaries, and opinion articles. Scientific contributions are refereed and edited to international standards. The journal publishes articles for SABRAO members mainly. The Journal preferred strongly that at least one author should be a current member of the Society. Non-members may also publish in the journal.
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