M. Cuellar-Herrera , L. Rocha , N. Saleh-Subaie , F. Velasco , G. Aguado-Carrillo , M. Alonso-Vanegas , R. Guevara-Guzmán , A.L. Velasco
{"title":"颞叶癫痫患者新皮层颞叶组织中5-HT1A受体与g蛋白功能偶联的变化","authors":"M. Cuellar-Herrera , L. Rocha , N. Saleh-Subaie , F. Velasco , G. Aguado-Carrillo , M. Alonso-Vanegas , R. Guevara-Guzmán , A.L. Velasco","doi":"10.1016/j.hgmx.2017.07.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The 5-hydroxytryptamine-1A receptors are known to be involved in the inhibition of seizures in epilepsy.</p></div><div><h3>Objective</h3><p>The main aim of the present study is to determine the functional coupling to G-protein of the 5-HT<sub>1A</sub> receptor through 8-OH-DPAT-stimulated [<sup>35</sup>S]GTPγS binding assay in lateral temporal neocortex tissue of patients with mesial temporal lobe epilepsy (mTLE) and correlate it with clinical data.</p></div><div><h3>Material and methods</h3><p>The activation of the G protein complex was determined by [<sup>35</sup>S]GTPγS binding assay. The temporal neocortex tissue was obtained from 5 patients with mTLE during epilepsy surgery and from 5 subjects (autopsies) who died due to an accident and without history of neurological disease.</p></div><div><h3>Results</h3><p>We found that values of maximal stimulation (<em>E</em><sub>max</sub>) of [<sup>35</sup>S]GTPγS binding significant decreased in the mTLE group (30.8%, <em>p</em> <!--><<!--> <!-->.05) when compared to the autopsy samples (65.9%). The values of potency (pEC<sub>50</sub>) were similar in both groups. However, we found no significant differences between the <em>E</em><sub>max</sub> and age of patient, age of seizure onset, duration of epilepsy and frequency of seizures. Also, the pEC<sub>50</sub> values revealed no significant correlations with the clinical data.</p></div><div><h3>Conclusions</h3><p>Our data provide evidence that the lateral temporal neocortex of patients with pharmacoresistant mTLE presents alterations in the functional coupling to G-protein of the 5-HT<sub>1A</sub> receptor. Such a change could be involved in hyperexcitability in the neocortex of patients with mTLE.</p></div>","PeriodicalId":31559,"journal":{"name":"Revista Medica del Hospital General de Mexico","volume":"81 4","pages":"Pages 177-183"},"PeriodicalIF":0.0000,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.hgmx.2017.07.002","citationCount":"0","resultStr":"{\"title\":\"Changes in functional coupling of 5-HT1A receptor to the G-protein in neocortex temporal tissues of patients with temporal lobe epilepsy\",\"authors\":\"M. Cuellar-Herrera , L. Rocha , N. Saleh-Subaie , F. Velasco , G. Aguado-Carrillo , M. Alonso-Vanegas , R. Guevara-Guzmán , A.L. Velasco\",\"doi\":\"10.1016/j.hgmx.2017.07.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The 5-hydroxytryptamine-1A receptors are known to be involved in the inhibition of seizures in epilepsy.</p></div><div><h3>Objective</h3><p>The main aim of the present study is to determine the functional coupling to G-protein of the 5-HT<sub>1A</sub> receptor through 8-OH-DPAT-stimulated [<sup>35</sup>S]GTPγS binding assay in lateral temporal neocortex tissue of patients with mesial temporal lobe epilepsy (mTLE) and correlate it with clinical data.</p></div><div><h3>Material and methods</h3><p>The activation of the G protein complex was determined by [<sup>35</sup>S]GTPγS binding assay. The temporal neocortex tissue was obtained from 5 patients with mTLE during epilepsy surgery and from 5 subjects (autopsies) who died due to an accident and without history of neurological disease.</p></div><div><h3>Results</h3><p>We found that values of maximal stimulation (<em>E</em><sub>max</sub>) of [<sup>35</sup>S]GTPγS binding significant decreased in the mTLE group (30.8%, <em>p</em> <!--><<!--> <!-->.05) when compared to the autopsy samples (65.9%). The values of potency (pEC<sub>50</sub>) were similar in both groups. However, we found no significant differences between the <em>E</em><sub>max</sub> and age of patient, age of seizure onset, duration of epilepsy and frequency of seizures. Also, the pEC<sub>50</sub> values revealed no significant correlations with the clinical data.</p></div><div><h3>Conclusions</h3><p>Our data provide evidence that the lateral temporal neocortex of patients with pharmacoresistant mTLE presents alterations in the functional coupling to G-protein of the 5-HT<sub>1A</sub> receptor. Such a change could be involved in hyperexcitability in the neocortex of patients with mTLE.</p></div>\",\"PeriodicalId\":31559,\"journal\":{\"name\":\"Revista Medica del Hospital General de Mexico\",\"volume\":\"81 4\",\"pages\":\"Pages 177-183\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.hgmx.2017.07.002\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Medica del Hospital General de Mexico\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0185106318300398\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Medica del Hospital General de Mexico","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0185106318300398","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Changes in functional coupling of 5-HT1A receptor to the G-protein in neocortex temporal tissues of patients with temporal lobe epilepsy
Background
The 5-hydroxytryptamine-1A receptors are known to be involved in the inhibition of seizures in epilepsy.
Objective
The main aim of the present study is to determine the functional coupling to G-protein of the 5-HT1A receptor through 8-OH-DPAT-stimulated [35S]GTPγS binding assay in lateral temporal neocortex tissue of patients with mesial temporal lobe epilepsy (mTLE) and correlate it with clinical data.
Material and methods
The activation of the G protein complex was determined by [35S]GTPγS binding assay. The temporal neocortex tissue was obtained from 5 patients with mTLE during epilepsy surgery and from 5 subjects (autopsies) who died due to an accident and without history of neurological disease.
Results
We found that values of maximal stimulation (Emax) of [35S]GTPγS binding significant decreased in the mTLE group (30.8%, p < .05) when compared to the autopsy samples (65.9%). The values of potency (pEC50) were similar in both groups. However, we found no significant differences between the Emax and age of patient, age of seizure onset, duration of epilepsy and frequency of seizures. Also, the pEC50 values revealed no significant correlations with the clinical data.
Conclusions
Our data provide evidence that the lateral temporal neocortex of patients with pharmacoresistant mTLE presents alterations in the functional coupling to G-protein of the 5-HT1A receptor. Such a change could be involved in hyperexcitability in the neocortex of patients with mTLE.
期刊介绍：
The Medical Journal of the Hospital General de Mexico is the official organ of the Medical Society of the Hospital General de Mexico. The journal accepts articles in Spanish or in English on the field of hospital medicine. The journal publishes original articles, clinical cases, reviews articles, history notes, issues on medical education, short communications and editorials at the invitation of the Society. All articles are double blind peer reviewed by at least 2 reviewers and finally classified as accepted or rejected by the Editorial Board.
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