果胶调节铅中毒大鼠血液生化参数、组织病理学、氧化应激生物标志物、细胞因子和hepcidn基因表达

IF 0.3 4区农林科学 Q4 VETERINARY SCIENCES

Slovenian Veterinary Research Pub Date : 2022-12-30 DOI:10.26873/svr-1591-2022

S. El-Bahr, S. Al-Sultan, Ahlam F Hamouda, S. Atwa, Seham Y. Abo kora, A. Amin, S. Shousha, S. Alhojaily, Aymmen Alnehas, Rabab R. Elzogby

{"title":"果胶调节铅中毒大鼠血液生化参数、组织病理学、氧化应激生物标志物、细胞因子和hepcidn基因表达","authors":"S. El-Bahr, S. Al-Sultan, Ahlam F Hamouda, S. Atwa, Seham Y. Abo kora, A. Amin, S. Shousha, S. Alhojaily, Aymmen Alnehas, Rabab R. Elzogby","doi":"10.26873/svr-1591-2022","DOIUrl":null,"url":null,"abstract":"Publications concerning the protective effect of pectin against lead induced toxicity in rats are not available. In order to study such effect, 40 male rats were divided into 3 groups. The first group was contained 10 rats that kept as control group. The second group was contained 10 rats that received pectin at dose of 100 mg/kg BW during experimental period (8 weeks). The third group was contained 20 rats that received 400mg/kg BW of lead acetate daily for 4 weeks then divided into two subgroups (3A and 3B). Subgroup 3A contained 10 rats that still receive lead acetate in the same dosage whereas, subgroup B co-treated with lead acetate and pectin daily for another 4 weeks. Blood samples were collected after 2, 4 and 8 weeks from the start of the experiment. Liver, kidney and bone marrows were collected only at the end of the experiment. Lead acetate induced anemia only after 4 weeks of administration as reflected on decreased values of Hb, PCV, MCV, MCH and MCHC. These indices remained at lower levels in lead acetate treated groups until the end of the experiment. Concentrations of serum ferritin, iron, total antioxidant capacity (TAC) and reduced glutathione (GSH) and the expression of hepatic hepcidin gene were decreased significantly in lead acetate intoxicated rats compared to control. Activities of ALT and AST and concentrations of urea, creatinine, Nitric oxide (NO), TNF-α, IL-6, total iron binding capacity (TIBC) and lead were increased significantly in lead acetate intoxicated group compared to control. Hepatic degeneration and hemorrhage, renal lytic necrosis and apoptosis of myeloid cells were most prominent changes in lead intoxicated rats. Lead acetated related changes were improved by co-treatment with pectin however; normal control values have not been achieved. Conclusively, pectin is recommended to protect against lead acetate toxicity in rats.\nKey words: lead acetate; toxicity; pectin; hepcidin; oxidative stress biomarkers; histopathology","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.3000,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PECTIN MODULATES HEMATO-BIOCHEMICAL PARAMETER, HISTOPATHOLOGY, OXIDATIVE STRESS BIOMARKERS, CYTOKINES AND EXPRESSION OF HEPCIDN GENE IN LEAD INTOXICATED RATS\",\"authors\":\"S. El-Bahr, S. Al-Sultan, Ahlam F Hamouda, S. Atwa, Seham Y. Abo kora, A. Amin, S. Shousha, S. Alhojaily, Aymmen Alnehas, Rabab R. Elzogby\",\"doi\":\"10.26873/svr-1591-2022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Publications concerning the protective effect of pectin against lead induced toxicity in rats are not available. In order to study such effect, 40 male rats were divided into 3 groups. The first group was contained 10 rats that kept as control group. The second group was contained 10 rats that received pectin at dose of 100 mg/kg BW during experimental period (8 weeks). The third group was contained 20 rats that received 400mg/kg BW of lead acetate daily for 4 weeks then divided into two subgroups (3A and 3B). Subgroup 3A contained 10 rats that still receive lead acetate in the same dosage whereas, subgroup B co-treated with lead acetate and pectin daily for another 4 weeks. Blood samples were collected after 2, 4 and 8 weeks from the start of the experiment. Liver, kidney and bone marrows were collected only at the end of the experiment. Lead acetate induced anemia only after 4 weeks of administration as reflected on decreased values of Hb, PCV, MCV, MCH and MCHC. These indices remained at lower levels in lead acetate treated groups until the end of the experiment. Concentrations of serum ferritin, iron, total antioxidant capacity (TAC) and reduced glutathione (GSH) and the expression of hepatic hepcidin gene were decreased significantly in lead acetate intoxicated rats compared to control. Activities of ALT and AST and concentrations of urea, creatinine, Nitric oxide (NO), TNF-α, IL-6, total iron binding capacity (TIBC) and lead were increased significantly in lead acetate intoxicated group compared to control. Hepatic degeneration and hemorrhage, renal lytic necrosis and apoptosis of myeloid cells were most prominent changes in lead intoxicated rats. Lead acetated related changes were improved by co-treatment with pectin however; normal control values have not been achieved. Conclusively, pectin is recommended to protect against lead acetate toxicity in rats.\\nKey words: lead acetate; toxicity; pectin; hepcidin; oxidative stress biomarkers; histopathology\",\"PeriodicalId\":21765,\"journal\":{\"name\":\"Slovenian Veterinary Research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2022-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Slovenian Veterinary Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.26873/svr-1591-2022\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Slovenian Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.26873/svr-1591-2022","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

关于果胶对大鼠铅中毒的保护作用的出版物还没有。为了研究这种作用，将40只雄性大鼠分为3组。第一组10只大鼠作为对照组。第二组大鼠10只，在实验期间(8周)给予100 mg/kg BW剂量的果胶。第三组20只大鼠，每天给予400mg/kg BW醋酸铅灌胃，连续4周，分为3A和3B两个亚组。3A亚组10只大鼠仍给予相同剂量的醋酸铅治疗，B亚组每天给予醋酸铅和果胶联合治疗，持续4周。实验开始后2周、4周和8周分别采集血样。肝、肾和骨髓只在实验结束时采集。通过Hb、PCV、MCV、MCH和MCHC的降低可以看出，醋酸铅仅在给药4周后才引起贫血。乙酸铅处理组在试验结束前这些指标均保持在较低水平。与对照组相比，乙酸铅中毒大鼠血清铁蛋白、铁、总抗氧化能力(TAC)、还原性谷胱甘肽(GSH)浓度及肝脏hepcidin基因表达均显著降低。与对照组相比，乙酸铅中毒组血清ALT、AST活性显著升高，尿素、肌酐、一氧化氮(NO)、TNF-α、IL-6、总铁结合能力(TIBC)和铅浓度显著升高。肝变性出血、肾溶解性坏死和髓细胞凋亡是铅中毒大鼠最显著的变化。而与果胶共处理可改善铅醋酸化相关变化;正常控制值尚未达到。最后，推荐果胶保护大鼠免受醋酸铅中毒。关键词:醋酸铅;毒性;果胶;hepcidin;氧化应激生物标志物;组织病理学

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

PECTIN MODULATES HEMATO-BIOCHEMICAL PARAMETER, HISTOPATHOLOGY, OXIDATIVE STRESS BIOMARKERS, CYTOKINES AND EXPRESSION OF HEPCIDN GENE IN LEAD INTOXICATED RATS

Publications concerning the protective effect of pectin against lead induced toxicity in rats are not available. In order to study such effect, 40 male rats were divided into 3 groups. The first group was contained 10 rats that kept as control group. The second group was contained 10 rats that received pectin at dose of 100 mg/kg BW during experimental period (8 weeks). The third group was contained 20 rats that received 400mg/kg BW of lead acetate daily for 4 weeks then divided into two subgroups (3A and 3B). Subgroup 3A contained 10 rats that still receive lead acetate in the same dosage whereas, subgroup B co-treated with lead acetate and pectin daily for another 4 weeks. Blood samples were collected after 2, 4 and 8 weeks from the start of the experiment. Liver, kidney and bone marrows were collected only at the end of the experiment. Lead acetate induced anemia only after 4 weeks of administration as reflected on decreased values of Hb, PCV, MCV, MCH and MCHC. These indices remained at lower levels in lead acetate treated groups until the end of the experiment. Concentrations of serum ferritin, iron, total antioxidant capacity (TAC) and reduced glutathione (GSH) and the expression of hepatic hepcidin gene were decreased significantly in lead acetate intoxicated rats compared to control. Activities of ALT and AST and concentrations of urea, creatinine, Nitric oxide (NO), TNF-α, IL-6, total iron binding capacity (TIBC) and lead were increased significantly in lead acetate intoxicated group compared to control. Hepatic degeneration and hemorrhage, renal lytic necrosis and apoptosis of myeloid cells were most prominent changes in lead intoxicated rats. Lead acetated related changes were improved by co-treatment with pectin however; normal control values have not been achieved. Conclusively, pectin is recommended to protect against lead acetate toxicity in rats. Key words: lead acetate; toxicity; pectin; hepcidin; oxidative stress biomarkers; histopathology

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Slovenian Veterinary Research VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： SLOVENIAN VETERINARY RESEARCH (ISSN 1580-4003) publishes original articles, which report the results of original research in most areas of biomedicine. The journal also publishes review articles dealing with rapidly developing areas of biomedicine or which update understanding of classical fields of biomedicine, as well as case reports, shorter scientific contributions, letters to the editor, etc.; which have not been published or are under consideration for publication elsewhere. Only papers written in English can be considered.