高粱WRKY转录因子SbWRKY45在干旱胁迫下促进转基因拟南芥种子发芽

IF 1.5 4区生物学 Q3 PLANT SCIENCES

Turkish Journal of Botany Pub Date : 2023-01-01 DOI:10.55730/1300-008x.2741

E. Baillo, M. S. Hanif, P. Xu, Zhengbin Zhang

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引用次数: 0

摘要

WRKY转录因子(WRKY transcription factors, TFs)家族是植物特异性TFs的一个重要家族，在各种非生物和生物胁迫反应中发挥重要作用。WRKY TFs因其在应激反应中的重要作用而受到广泛关注。然而，它们在高粱(高粱双色)中的功能却相对滞后。本研究从高粱中分离到一个WRKY基因SbWRKY45，并对其进行了鉴定。SbWRKY45属于IIa族，由1个内含子和2个外显子组成，编码430个氨基酸。SbWRKY45位于4号染色体上。顺式元件预测分析表明，SbWRKY45的启动子区域存在多个非生物胁迫相关元件。qRT-PCR结果显示，盐胁迫下SbWRKY45在干旱和寒冷条件下表达量显著上调，在热胁迫下表达量变化显著，诱导程度较弱。SbWRKY45在高粱叶、茎、根等不同组织中均表现出对胁迫的响应。组织特异性表达模式显示，与叶和茎相比，SbWRKY45在根中的表达量较高，表明SbWRKY45可能在根中发挥重要作用。在干旱胁迫下，SbWRKY45的过表达提高了转基因拟南芥的发芽率，促进了根的生长。综上所述，SbWRKY45可能参与了介导高粱对干旱胁迫的响应，并在高粱的非生物胁迫响应中发挥重要作用。

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Sorghum WRKY transcription factor SbWRKY45 enhanced seed germination under drought stress in transgenic Arabidopsis

: The WRKY transcription factors (TFs) family is an important family of plant-specific TFs, playing vital roles in various abiotic and biotic stress responses. WRKY TFs are gaining considerable attention due to their significant roles in stress responses. However, their functions in sorghum ( Sorghum bicolor ) are lagging behind. In this study, a WRKY gene designated as SbWRKY45 was isolated and characterized from sorghum. SbWRKY45 , belonging to Group IIa, consists of one intron and two exons and encodes 430 amino acids. SbWRKY45 is located in chromosome 4. The cis -element prediction analysis showed that the promoter region of SbWRKY45 has several abiotic stress-associated elements. The qRT-PCR results showed that SbWRKY45 was significantly up-regulated in response to drought and cold under salt treatments, was notably changed, and was induced weakly under heat stress. SbWRKY45 exhibited a response to stress in different sorghum tissues, including leaves, stems, and roots. A tissue-specific expression pattern showed that SbWRKY45 was highly expressed in roots compared with leaves and stems, suggesting that SbWRKY45 may play an important role in roots. Overexpression of SbWRKY45 increased germination rates and promoted root growth in transgenic Arabidopsis under drought stress. Taken together, our findings indicate that SbWRKY45 may be involved in mediating the response to drought stress and play a vital role in the abiotic stress response of sorghum.

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来源期刊

Turkish Journal of Botany PLANT SCIENCES-

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Botany is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts manuscripts (in English) covering all areas of plant biology (including genetics, evolution, systematics, structure, function, development, diversity, conservation biology, biogeography, paleobotany, ontogeny, functional morphology, ecology, reproductive biology, and pollination biology), all levels of organisation (molecular to ecosystem), and all plant groups and allied organisms (algae, fungi, and lichens). Authors are required to frame their research questions and discuss their results in terms of major questions in plant biology. In general, papers that are too narrowly focused, purely descriptive, or broad surveys, or that contain only preliminary data or natural history, will not be considered (*). The following types of article will be considered: 1. Research articles: Original research in various fields of botany will be evaluated as research articles. 2. Research notes: These include articles such as preliminary notes on a study or manuscripts on the morphological, anatomical, cytological, physiological, biochemical, and other properties of plant, algae, lichen and fungi species. 3. Reviews: Reviews of recent developments, improvements, discoveries, and ideas in various fields of botany. 4. Letters to the editor: These include opinions, comments relating to the publishing policy of the Turkish Journal of Botany, news, and suggestions. Letters should not exceed one journal page. (*) 1. Raw floristic lists (of algae, lichens, fungi, or plants), species descriptions, chorological studies, and plant sociology studies without any additional independent approaches. 2. Comparative morphology and anatomy studies (that do not cover a family, tribe, subtribe, genus, subgenus, section, subsection, or species complexes with taxonomical problems) without one or more independent additional approaches such as phylogenetical, micromorphological, chromosomal and anatomical analyses. 3. Revisions of family, tribe, genus, subgenus, section, subsection, or species complexes without any original outputs such as taxonomical status changes, IUCN categories, and phenological and ecological analyses. 4. New taxa of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 3 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms. 5. New taxa of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 5 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms.