伊朗一种与红花结扎有关的植物原体的分子研究

Acta Phytopathologica Et Entomologica Hungarica Pub Date : 2019-12-01 DOI:10.5958/2249-4677.2019.00014.8

Hadi Mahmoudi, M. Salari, M. G. Zamharir, M. Ghorbani

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引用次数: 2

摘要

2017-2018年，在南呼罗珊（伊朗）的田地里观察到一种红花病。受影响的植物具有广泛的筋膜化、浓密的生长、小叶、叶序和缩短的节间。使用通用植原体引物对P1/P7、R16mF1/mR1和M1/M2进行聚合酶链式反应（PCR）测定，以检测推定的植原体。巢式PCR分析显示，所有有症状的植物都对植原体感染呈阳性，而无症状的植物则呈阴性。PCR扩增的16S rDNA序列的核苷酸序列分析和RFLP分析表明，伊朗红花筋膜化与一种属于花生丛属植物原体（16SrII）有关。系统发育分析证实，红花筋膜植原体（SaP）必须归入“Candidatus phytoplasma aurantifolia”（16SrII）分类群。据我们所知，这是伊朗和世界上第一份与红花筋膜相关的植原体的文献。

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Molecular study of a phytoplasma associated with safflower fasciation in Iran

A safflower disease was observed in the fields of South Khorasan (Iran) in 2017–2018. Affected plants had extensive fasciation, bushy growth, small leaves, phyllody and shortened internodes. Polymerase chain reaction (PCR) assays were performed using universal phytoplasma primers pairs P1/P7, R16mF1/mR1 and M1/ M2 to detect putative phytoplasma (s). Nested PCR analyses showed that all symptomatic plants were positive for phytoplasma infection, while asymptomatic plants were negative. Nucleotide sequence analysis and RFLP analyses of PCR-amplified 16S rDNA sequences indicated that safflower fasciation in Iran was associated with a phytoplasma that belonged to the peanut witches’ broom group (16SrII). Phylogenetic analysis confirmed that safflowers fasciation phytoplasma (SaP) must be classified in ‘ Candidatus phytoplasma aurantifolia’ (16SrII) taxonomic group. To our knowledge, this is the first documentation of a phytoplasma associated with safflower fasciation in Iran and the world.

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来源期刊

Acta Phytopathologica Et Entomologica Hungarica Agricultural and Biological Sciences-Plant Science

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The journal publishes papers on the infectious diseases of plants, damages caused by insects and deals with the basic aspects of chemical and biological protection. Within its field of interest additional topics such as resistance against plant diseases, and physiological, biochemical and molecular questions of plant resistance and susceptibility are also discussed. Publishes book reviews and advertisements.