两个非洲菊品种的比较转录组分析揭示了其抗寒机制

Q3 Agricultural and Biological Sciences
Shen-chong Li, Qinli Shan, Rongpei Yu, A. N. Siddique, Xia Li, Chunmei Yang, Jiwei Ruan, Mingyang Li
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引用次数: 5

摘要

温度是决定非洲菊分布和生长模式的核心因素之一。该物种的品种在形态和胁迫适应方面有很大的差异。在本研究中,使用Illumina Hiseq2000测序平台,对两个具有鉴别表型的单倍体品种,即红吉星非洲菊和秋日非洲菊进行了比较转录组分析,以揭示差异表达的功能性单基因。通过高通量cDNA测序(RNA-seq),我们从19.09G的初级读数中产生了99160个UniGenes,平均长度为978bp,N50等于1620。它由Trimmomatic过滤并由Trinity组装。通过BLASTX定位到五个蛋白质数据库,共有58127个转录物进行了功能注释,其中最高的差异表达单基因(DEG)指向转录调控、二次代谢和应激反应等。在智能冷却培养箱中冷处理(-2°C)单倍体幼苗对两个品种都有不利影响,秋日表现出较低的叶绿素荧光。这表明红吉星具有较好的抗寒性。qRT-PCR结果表明,除常见途径外,胶乳生物合成等新途径可能有助于红吉星提高抗寒性。鉴于低温的普遍性,本研究为冷应力状态的机制解剖和指导提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative transcriptome analysis of two Gerbera hybrida cultivars revealed cold tolerance mechanism
Temperature is one of central factors that shape the distribution and growth pattern of Gerbera hybrida. The cultivars of this species have vast variation in the morphology and stress adaptation. In the current study, comparative transcriptome analysis of two haploid cultivars with discriminative phenotype, i.e., Gerbera hybrida Hongjixing and Gerbera hybrida Qiuri, was used to uncover differentially expressed functional unigenes using an Illumina Hiseq2000 sequencing platform. Through high-throughput cDNA sequencing (RNA-seq), we generated 99160 UniGenes from 19.09G primary reads with an average length of 978 bp and an N50 equaling 1620. It was filtered by Trimmomatic and assembled by Trinity. A total of 58127 transcripts were functional annotated by mapping to five protein databases by BLASTX, with topmost differently expressed unigenes (DEGs) directed to transcriptional regulation, secondary metabolism and stress response, etc. Cold treatment (-2°C) of haploid seedlings in intelligent cooled incubator had adverse effects on both cultivars, which was more serious in Qiuri which was exhibited by the lower chlorophyll fluorescence after cold treatment, compared to Hongjixing. This indicates the better cold tolerance of Hongjixing. The qRT-PCR result indicated that besides common pathways, novel pathways like latex biosynthesis potentially contributes to the better cold tolerance of Hongjixing. In view of the ubiquity of cold temperature, this study provided new insights into the mechanism dissection and guidance for cold stress condition.
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来源期刊
Plant Omics
Plant Omics 生物-植物科学
CiteScore
1.30
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.
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