脊髓神经结扎大鼠引起背根神经节神经性疼痛的反应多发生在早期

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2022-09-01 DOI:10.1016/j.angen.2022.200138

Chunmei Wang, Xiaofang Lin, Wei Tang, Dongmei Wang

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引用次数: 0

摘要

为了研究早期和晚期神经性疼痛的机制，采用核糖核酸测序(RNA-seq)技术对脊髓神经结扎(SNL)诱导大鼠背根神经节(DRG)转录组进行了分析。研究发现，导致背根神经节神经性疼痛的促炎反应、免疫反应等反应大多发生在早期。早期有378个基因上调，42个基因下调，晚期有27个基因上调，23个基因下调。4个新的疼痛相关基因被发现:Cd8a, Bub1b, Ccna2和Cdk1。从京都基因基因组百科(KEGG)通路和差异表达基因(DEGs)富集的基因本体(GO)功能分析，背根神经节在早期应对周围神经损伤时应进行免疫、促炎及相关代偿调节。晚期以维持长期或慢性炎症为特征。

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Most reactions leading to neuropathic pain at dorsal root ganglion of rats with spinal nerve ligation have occurred in the early phase

In order to study the mechanism of early and late neuropathic pain, Ribonucleic acid sequencing (RNA-seq) technique was used to analyze the dorsal root ganglion (DRG) transcriptome of rat induced by spinal nerve ligation (SNL). It was found that most of the reactions leading to neuropathic pain at dorsal root ganglion, such as pro-inflammatory response and immunity, occurred in the early phase.378 genes are up-regulated and 42 genes are down-regulated in the early phase, while in the late phase, there are 27 up-regulated genes and 23 down-regulated genes. Four new pain related genes are found: Cd8a, Bub1b, Ccna2 and Cdk1. From the analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and Gene Ontology (GO) functions enriched by differentially expressed genes (DEGs), dorsal root ganglion should carry out immunities, pro-inflammations and related compensatory regulation in response to peripheral nerve injury in the early phase. The late phase is characterized by maintaining long-term or chronic inflammation.

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来源期刊

Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.