利用线粒体DNA序列分析比较埃及本地兔与西班牙普通兔的地位和来源

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2020-06-30 DOI:10.4995/wrs.2020.12219

A. Emam, S. Afonso, P. González-Redondo, G. Mehaisen, A. Azoz, N. A. Ahmed, N. Fernand

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引用次数: 5

摘要

利用线粒体DNA (mtDNA)和细胞色素b (cyt b)基因序列测定了埃及和西班牙132个地方家兔品种的遗传多样性和系统发育状况。埃及当地的兔子品种是埃及红巴拉迪(ERB)、埃及黑巴拉迪(EBB)和埃及Gabali Sinai (EGS)。然而，西班牙地方兔品种是西班牙普通兔(SCR)。将以前的品种与取自西班牙阿尔巴塞特的欧洲野兔(EWR)进行比较。本研究共确定了353个突变，290个多态性位点，14个单倍型，0.06126个单倍型多样性和-1.900个(P<0.05)的田岛氏病。83.3%的埃及兔和11.7%的EWR兔多为A型单倍型，63.8%的西班牙兔和36.2%的EGS兔多为B型单倍型。本研究利用已发表的47个家养和野生小丘圆齿兔的基因序列，对所测家兔品种的来源和亲缘关系进行了分析。最常见的单倍型(A)与44.7%的已发表序列相结合。单倍型B的配合率为8.5%。埃及、SCR和EWR单倍型分散在集群1中，而EGS单倍型和EWR单倍型分别在集群2和集群2中。我们的结果假设ERB、EBB和SCR的遗传多样性很低。埃及品种和SCR是从欧洲兔引进的。我们发现ERB和EBB属于一个品种。

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Status and origin of Egyptian local rabbits in comparison with Spanish common rabbits using mitochondrial DNA sequence analysis

Mitochondrial DNA (mtDNA) and cytochrome b (cyt b) gene sequences were used to determine the status of genetic diversity and phylogeny for 132 individuals from local rabbit breeds in Egypt and Spain. The Egyptian local rabbit breeds were Egyptian Red Baladi (ERB), Egyptian Black Baladi (EBB) and Egyptian Gabali Sinai (EGS). However, the Spanish local rabbit breed was Spanish common rabbit (SCR). Previous breeds were compared with European Wild Rabbit taken from Albacete, Spain (EWR). A total of 353 mutations, 290 polymorphic sites, 14 haplotypes, 0.06126 haplotype diversity and –1.900 (P<0.05) for Tajima’s D were defined in this study. Haplotype A mostly occurred in 83.3% of Egyptian rabbits and 11.7 % of EWR, while haplotype B occurred in 63.8% of Spanish rabbits and 36.2% of the EGS breed. A total of 47 domestic and wild Oryctolagus cuniculus published sequences were used to investigate the origin and relation among the rabbit breeds tested in this study. The most common haplotype (A) was combined with 44.7% of published sequences. However, haplotype B was combined with 8.5%. Haplotypes of Egyptian, SCR and EWR were scattered in cluster 1, while we found only one EGS haplotype with two haplotypes of EWR in cluster 2. Our results assumed that genetic diversity for ERB, EBB and SCR was very low. Egyptian breeds and SCR were introduced from European rabbits. We found that ERB and EBB belong to one breed.

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来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.