Deep Immune Status Monitoring with Multi-color Flow Cytometry

With the development of immune therapy, the role of immune monitoring during treatments has been emphasized. Flow cytometry is the best method for immune status monitoring because of its characteristic of multi-parameter analysis. To broadly monitor immune status, we established and validated a multi-color comprehensive panel of 29 antibodies to cover 90 immune cell subsets and the research work was published in the issue of ILSH recently [1]. The article reported that the clinical applications of 10-color flow cytometry to monitor the immune status of patients before or after clinical treatments in 2 ml whole blood samples.