{"title":"骨髓间充质干细胞治疗大鼠慢性胰腺炎的实验研究","authors":"Xin Wang, Yue Du, Zhao Li","doi":"10.3760/CMA.J.ISSN.1007-631X.2020.01.015","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effects and mechanisms of BMSCs on CP in rats. \n \n \nMethods \n40 SD rats were divided into 4 groups with 10 in each: control group, model group, treatment group, and sham treatment group.The pancreatic fibrosis and pathological score were evaluated.The expression of α-SMA, collagen type I and III, IL-10 in pancreatic tissue were detected by ELASA. The expression of apoptosis genes BNIP3 in pancreatic tissue was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway in pancreatic tissue was determined by Western-blot method. The culture system of PSC was divided into 2 groups with 5 in each: control group and treatment group.The expression of α-SMA, collagen type I and III, IL-10 for PSC were detected by ELASA. The expression of apoptosis genes BNIP3 for PSC was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway for PSC was determined by Western-blot method. \n \n \nResults \n(1)The pancreatic fibrosis and pathological score in treatment group were lower than model group and sham treatment group (P<0.05). The expression of α-SMA, collagen type I and III in tissues were less in treatment group compared with model group and sham treatment group (P<0.05) while the level of BNIP3 and IL-10 in pancreatic tissue were higher in treatment group compared with model group and sham treatment group with significant group(P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were lower in treatment group compared with model group and sham treatment group (P<0.05). (2) The expression of α-SMA, collagen type I and III were less in treatment group compared with control group (P<0.05). The level of BNIP3 and IL-10 were higher in treatment group compared (P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were less in treatment group compared with control group (P<0.05). \n \n \nConclusion \nBMSCs reduce the activation and proliferation of PSC and then lower pancreatic fibrosis degree in rats with chronic pancreatitis. \n \n \nKey words: \nPancreatitis, chronic; Mesenchymal stem cells; Stellate cells","PeriodicalId":66425,"journal":{"name":"中华普通外科杂志","volume":"35 1","pages":"52-56"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The experimental study of bone marrow mesenchymal stem cells for the treatment of chronic pancreatitis in rats\",\"authors\":\"Xin Wang, Yue Du, Zhao Li\",\"doi\":\"10.3760/CMA.J.ISSN.1007-631X.2020.01.015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effects and mechanisms of BMSCs on CP in rats. \\n \\n \\nMethods \\n40 SD rats were divided into 4 groups with 10 in each: control group, model group, treatment group, and sham treatment group.The pancreatic fibrosis and pathological score were evaluated.The expression of α-SMA, collagen type I and III, IL-10 in pancreatic tissue were detected by ELASA. The expression of apoptosis genes BNIP3 in pancreatic tissue was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway in pancreatic tissue was determined by Western-blot method. The culture system of PSC was divided into 2 groups with 5 in each: control group and treatment group.The expression of α-SMA, collagen type I and III, IL-10 for PSC were detected by ELASA. The expression of apoptosis genes BNIP3 for PSC was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway for PSC was determined by Western-blot method. \\n \\n \\nResults \\n(1)The pancreatic fibrosis and pathological score in treatment group were lower than model group and sham treatment group (P<0.05). The expression of α-SMA, collagen type I and III in tissues were less in treatment group compared with model group and sham treatment group (P<0.05) while the level of BNIP3 and IL-10 in pancreatic tissue were higher in treatment group compared with model group and sham treatment group with significant group(P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were lower in treatment group compared with model group and sham treatment group (P<0.05). (2) The expression of α-SMA, collagen type I and III were less in treatment group compared with control group (P<0.05). The level of BNIP3 and IL-10 were higher in treatment group compared (P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were less in treatment group compared with control group (P<0.05). \\n \\n \\nConclusion \\nBMSCs reduce the activation and proliferation of PSC and then lower pancreatic fibrosis degree in rats with chronic pancreatitis. \\n \\n \\nKey words: \\nPancreatitis, chronic; Mesenchymal stem cells; Stellate cells\",\"PeriodicalId\":66425,\"journal\":{\"name\":\"中华普通外科杂志\",\"volume\":\"35 1\",\"pages\":\"52-56\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华普通外科杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2020.01.015\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华普通外科杂志","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1007-631X.2020.01.015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The experimental study of bone marrow mesenchymal stem cells for the treatment of chronic pancreatitis in rats
Objective
To investigate the effects and mechanisms of BMSCs on CP in rats.
Methods
40 SD rats were divided into 4 groups with 10 in each: control group, model group, treatment group, and sham treatment group.The pancreatic fibrosis and pathological score were evaluated.The expression of α-SMA, collagen type I and III, IL-10 in pancreatic tissue were detected by ELASA. The expression of apoptosis genes BNIP3 in pancreatic tissue was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway in pancreatic tissue was determined by Western-blot method. The culture system of PSC was divided into 2 groups with 5 in each: control group and treatment group.The expression of α-SMA, collagen type I and III, IL-10 for PSC were detected by ELASA. The expression of apoptosis genes BNIP3 for PSC was assayed by qRT-PCR. The protein of TGF-β1, Smad2, Smad3 and Smad4 in TGF-β/Smad signal pathway for PSC was determined by Western-blot method.
Results
(1)The pancreatic fibrosis and pathological score in treatment group were lower than model group and sham treatment group (P<0.05). The expression of α-SMA, collagen type I and III in tissues were less in treatment group compared with model group and sham treatment group (P<0.05) while the level of BNIP3 and IL-10 in pancreatic tissue were higher in treatment group compared with model group and sham treatment group with significant group(P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were lower in treatment group compared with model group and sham treatment group (P<0.05). (2) The expression of α-SMA, collagen type I and III were less in treatment group compared with control group (P<0.05). The level of BNIP3 and IL-10 were higher in treatment group compared (P<0.05). The level of TGF-β1, Smad2, Smad3 and Smad4 were less in treatment group compared with control group (P<0.05).
Conclusion
BMSCs reduce the activation and proliferation of PSC and then lower pancreatic fibrosis degree in rats with chronic pancreatitis.
Key words:
Pancreatitis, chronic; Mesenchymal stem cells; Stellate cells
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