小麦（Triticum aestivum L.）千粒重QTL新DNA标记的建立与鉴定

IF 1.2 4区农林科学 Q3 AGRONOMY

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI:10.17221/35/2019-cjgpb

Congcong Yang, Jian Ma, Cong Li, Min-jie Sun, Yaya Zou, Ting Li, Y. Mu, H. Tang, X. Lan

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引用次数: 1

摘要

千粒重是影响小麦产量的重要性状。利用西藏半野生小麦Q1028 (Q1028)与正麦9023 (ZM9023)杂交构建的重组自交系(RIL)群体，在小麦2D染色体上发现了一个控制TGW的主要数量性状位点(QTL)。该QTL阳性等位基因来自ZM9023。为了进一步表征该QTL，在这里，我们试图开发和验证高分辨率熔化(HRM)和序列特征扩增区(SCAR)标记。1个HRM标记(0C98-411)和2个SCAR标记(E301-700和bbbb -10470)被开发并整合到遗传图谱中。这三个标记在三个不同遗传背景的群体中都得到了验证。0C98-411是与QTgw最密切相关的标记。分子标记辅助育种。

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The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.)

Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into the genetic map. All of these three markers were validated in three populations with different genetic backgrounds. 0C98-411 is the most closely linked marker that could trace QTgw.sau-2D in molecular marker assisted breeding.

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来源期刊

Czech Journal of Genetics and Plant Breeding Agricultural and Biological Sciences-Plant Science

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Original scientific papers, critical reviews articles and short communications from the field of theoretical and applied plant genetics, plant biotechnology and plant breeding. Papers are published in English.