Establishment of an ambient air sevoflurane measurement method using ECD-­GC

The experiments were performed using a gas chro­ matograph (GC 2014 ; Shimadzu Corporation, Kyoto, Japan) equipped with an electron capture detector (ECD) (Fig. 1), and a medium polar col­ umn (Rtx­200 ; Restek Corporation, Bellefronte, PA, USA) with chemically bonded trifluoropropyl methyl polysiloxane as a liquid phase. Experimental samples were prepared by adding sevoflurane into 1 L vacuum sampling bottles (GL Sciences, Tokyo, Japan) filled with indoor air using a micropipette (Eppendorf, Hamburg, Germany). After waiting for the sevoflurane (Maruishi Pharmaceutical, Osaka, Japan) volatilization at room temperature, 20 μL of the sample from the sampling bottle was injected into the gas chromatograph using a micro syringe (Terumo, Tokyo, Japan). Establishment of an ambient air sevoflurane measurement method using ECD­GC