双峰灰鼠肌钙蛋白C的cDNA克隆及表达分析(直翅目:灰鼠科)

IF 0.2 4区 农林科学 Q4 ENTOMOLOGY
Ji-Hye Song, Kisang Kwon, Nuri Lee, Hyojung Shin, Dong Woon Kim, Honggeun Kim, Ans O-Yu Kwon
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引用次数: 1

摘要

摘要:很少有报道描述饥饿对昆虫肌肉生理和发育的影响。本研究检测了肌钙蛋白C(TnC)在两种斑点蟋蟀(双斑蟋蟀;直翅目:蟋蟀科)饥饿和再喂养过程中在骨骼肌和消化道中的作用。双斑蝥的TnC基因被克隆并编码一个153个氨基酸的蛋白质,理论等电点为4.02,分子量为17368.23Da。同源性分析表明,GrybiTnC与其他昆虫的TnCs具有高度的氨基酸相似性,序列同源性在~54%至75%之间。GrybiTnC有两个Ca2+结合位点和五个EF手基序,与大多数TnC蛋白相似。在饥饿和再喂养条件下,在三个不同的骨骼肌和消化道区域检测GrybiTnC基因的表达。饥饿期间,GrybiTnC基因在肌肉中的表达没有变化,但在前肠和后肠中上调。GrybiTnC基因在肌肉中的表达在再喂养和饥饿条件下是相似的,但它在前肠和中肠通过再喂养碳水化合物和脂质而上调,在后肠通过再喂食碳水化合物、蛋白质和脂质而下调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
cDNA Cloning and Expression Analysis of Troponin C from Gryllus bimaculatus (Orthoptera: Gryllidae)
ABSTRACT: Few reports have described the effect of starvation on insect muscle physiology and development. This study examined the role of troponin C (TnC) in the skeletal muscles and digestive tracts of the two-spotted cricket (Gryllus bimaculatus; Orthoptera: Gryllidae) during starvation and re-feeding. The TnC gene of G. bimaculatus (GrybiTnC) was cloned and found to encode a 153-amino-acid protein with a theoretical isoelectric point of 4.02 and molecular weight of 17,368.23 Da. Homology analyses revealed that GrybiTnC exhibited high amino acid similarities with TnCs from other insects, showing sequence identities ranging from ∼54 to 75%. GrybiTnC has two Ca2+-binding sites and five EF-hand motifs, similar to most TnC proteins. GrybiTnC gene expression was examined under starvation and re-feeding conditions in three different skeletal muscle and digestive tract regions. During starvation, GrybiTnC gene expression was unchanged in muscle but upregulated in the foregut and hindgut. Whereas GrybiTnC gene expression in muscle was similar under re-feeding and starvation conditions, it was upregulated in the foregut and midgut by re-feeding with carbohydrates and lipids and downregulated in the hindgut by re-feeding with carbohydrates, proteins and lipids.
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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.
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