桑叶Kuwanon X对人腺病毒具有抗病毒活性

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-05-12 DOI:10.2217/fvl-2023-0001

Chi Li, Jikui Deng, Lifeng Qi

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引用次数: 0

摘要

目的：研究桑叶二苯乙烯衍生物的抗腺病毒活性。材料与方法：在人气道上皮细胞模型上检测腺病毒-3和-7的抗腺病毒活性。细胞毒性通过LDH测定进行评估。腺病毒DNA通过qPCR进行定量。结果：5种桑叶二苯乙烯衍生物均具有抗腺病毒活性，其中Kuwanon X的抑制作用最强。Kuwanon X在大浓度范围内没有表现出明显的细胞毒性。机制研究表明，Kuwanon X不会影响病毒进入和腺病毒基因组的核转位，但会减少病毒DNA的产生。结论：桑叶二苯乙烯类化合物库瓦农X是治疗腺病毒的良好候选药物。

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Kuwanon X from mulberry leaves exhibits antiviral activity against human adenoviruses

Aim: This study aimed to investigate the anti–adenoviral activity of stilbene derivatives from mulberry leaves. Materials & methods: The anti–adenoviral activity was tested against adenoviruses -3 and -7 on human airway epithelial cell models. Cytotoxicity was assessed by LDH assay. Adenoviral DNA was quantified by qPCR. Results: All five tested stilbene derivatives from mulberry leaves exhibited anti–adenoviral activity, with Kuwanon X showing the highest inhibitory effect. Kuwanon X showed no apparent cytotoxicity for a wide range of concentrations. The mechanistic study revealed that Kuwanon X did not affect viral entry and nuclear translocation of the adenoviral genome but reduced viral DNA production. Conclusion: Stilbene derivatives like Kuwanon X from mulberry leaves are good candidates for antiviral treatment against AdV.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.