大麻木槿(KENAF)离体繁殖的建立

Nor Syafawati Mohamad Pauzi, Nurul Ain Saipul Bahari, Z. Zainuddin
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引用次数: 1

摘要

木槿大麻或俗称红麻是一种多功能植物,可作为许多制造业和畜牧业的资源。红麻最初种植在西非,现在分布在包括马来西亚在内的许多国家,因为它的纤维被证明是汽车、造纸和生物复合材料等主要行业的最终替代资源。事实上,在马来西亚,由于其适应广泛的气候条件,红麻有可能被选为替代烟草的新型工业作物。由于过去几十年来对红麻的需求急剧增加,人们对红麻的微繁再生有了很多兴趣。因此,本研究旨在建立大麻大麻的体外繁殖体系。在添加不同浓度苯氨基嘌呤(BAP)的Murashige和Skoog (MS)培养基上诱导愈伤组织。结果表明,不同浓度的BAP均能诱导愈伤组织。诱导愈伤组织最健康、最大的最佳BAP浓度为3.0 mg/l。在MS培养基中添加不同组合和浓度的IBA、BA和GA3,尝试诱导愈伤组织的芽和根。7个处理中,3个处理成功诱导了芽的形成;处理T3 (MS + 1.0 mg/l IBA + 2.5 mg/l BA)、处理T5 (MS + 0.1 mg/l IBA + 2.0 mg/l BA + 0.3 mg/l GA3)和处理T6 (MS + 1.0 mg/l IBA + 2.5 mg/l BA + 0.3 mg/l GA3)。本研究结果可为大麻大麻组织培养的进一步研究奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ESTABLISHMENT OF IN VITRO PROPAGATION OF Hibiscus cannabinus (KENAF)
Hibiscus cannabinus or commonly known as kenaf is a versatile plant that serves as resources for numerous manufacturing and livestock industries. Originally planted in West Africa, kenaf is now distributed in many countries including Malaysia as its fibres were proved to be an ultimate alternative resource for major industries such as automotive, paper and bio-composite. In fact, in Malaysia, due to its adaptation to wide range of climatic conditions, kenaf has potentially be chosen as a new industrial crop replacing tobacco. There have been many interests on regenerating kenaf via micropropagation as the demand for this crop has been increasing tremendously since the past decades. Hence, this study is initiated with the objective to establish in vitro propagation system of H. cannabinus. The callus induction was achieved on Murashige and Skoog (MS) media supplemented with different concentrations of benzylaminopurine (BAP). It was observed that calli were successfully induced on all the BAP concentrations tested. The optimum concentration of BAP that induced the healthiest and biggest calli was 3.0 mg/l. Shoot and root induction from the calli were attempted using MS medium supplemented with different combinations and concentrations of IBA, BA and GA3. From the seven treatments, three treatments successfully induced formation of shoot; treatment T3 (MS + 1.0 mg/l IBA + 2.5 mg/l BA), treatment T5 (MS + 0.1 mg/l IBA + 2.0 mg/l BA + 0.3 mg/l GA3) and treatment T6 (MS + 1.0 mg/l IBA + 2.5 mg/l BA + 0.3 mg/l GA3). The results obtained in this study can paved for more research on tissue culture of H. cannabinus.
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