{"title":"生长因子递送系统对牙齿干细胞细胞活性的作用：系统综述（第二部分）。","authors":"Sayna Shamszadeh, Armin Shirvani, Saeed Asgary","doi":"10.2174/1574888X17666220609093939","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The current systematic review aims to provide the available <i>ex vivo</i> evidence evaluating the biological interactions of dental stem cells (DSCs) and growth factor delivery systems.</p><p><strong>Methods: </strong>Following the Preferred Reporting Items for a Systematic Reviews and Meta-Analyses (PRISMA) guidelines, systematic search was conducted in the electronic databases (PubMed/Medline, Scopus, Web of Science, and Google Scholar) up to January 2022. Studies evaluating the biological interactions of DSCs and growth factor delivery systems were included. The outcome measures were cell cytocompatibility, mineralization, and differentiation.</p><p><strong>Results: </strong>Sixteen studies were selected for the qualitative synthesis. The following growth factor delivery systems exhibit adequate cytocompatibility, enhanced mineralization, and osteo/odontoblast differentiation potential of DSCs: 1) Fibroblast growth factor (FGF-2)-loaded-microsphere and silk fibroin, 2) Bone morphogenic protein-2 (BMP-2)-loaded-microsphere and mesoporous calcium silicate scaffold, 3) Transforming growth factor Beta 1 (TGF-ß1)-loaded-microsphere, glass ionomer cement (GIC), Bio-GIC and liposome, 4) TGF-ß1-loaded-nanoparticles/scaffold, 5) Vascular endothelial growth factor (VEGF)-loaded-fiber and hydrogel, 6) TGF-ß1/VEGF-loaded-nanocrystalline calcium sulfate/hydroxyapatite/calcium sulfate, 7) Epidermal growth factor-loaded- nanosphere, 8) Stem cell factor/DSCs-loaded-hydrogel and Silk fibroin, 9) VEGF/BMP-2/DSCs-loaded-Three-dimensional matrix, 10) VEGF/DSCs-loaded-microsphere/hydrogel, and 11) BMP-2/DSCs and VEGF/DSCs-loaded-Collagen matrices. The included delivery systems showed viability, except for Bio-GIC on day 3. The choice of specific growth factors and delivery systems (<i>i.e.</i>, BMP-2-loaded-microsphere and VEGF-loaded-hydrogel) resulted in a greater gene expression.</p><p><strong>Conclusions: </strong>This study, with low-level evidence obtained from <i>ex vivo</i> studies, suggests that growth factor delivery systems induce cell proliferation, mineralization, and differentiation toward a therapeutic potential in regenerative endodontics.</p>","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Role of Growth Factor Delivery Systems on Cellular Activities of Dental Stem Cells: A Systematic Review (Part II).\",\"authors\":\"Sayna Shamszadeh, Armin Shirvani, Saeed Asgary\",\"doi\":\"10.2174/1574888X17666220609093939\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The current systematic review aims to provide the available <i>ex vivo</i> evidence evaluating the biological interactions of dental stem cells (DSCs) and growth factor delivery systems.</p><p><strong>Methods: </strong>Following the Preferred Reporting Items for a Systematic Reviews and Meta-Analyses (PRISMA) guidelines, systematic search was conducted in the electronic databases (PubMed/Medline, Scopus, Web of Science, and Google Scholar) up to January 2022. Studies evaluating the biological interactions of DSCs and growth factor delivery systems were included. The outcome measures were cell cytocompatibility, mineralization, and differentiation.</p><p><strong>Results: </strong>Sixteen studies were selected for the qualitative synthesis. The following growth factor delivery systems exhibit adequate cytocompatibility, enhanced mineralization, and osteo/odontoblast differentiation potential of DSCs: 1) Fibroblast growth factor (FGF-2)-loaded-microsphere and silk fibroin, 2) Bone morphogenic protein-2 (BMP-2)-loaded-microsphere and mesoporous calcium silicate scaffold, 3) Transforming growth factor Beta 1 (TGF-ß1)-loaded-microsphere, glass ionomer cement (GIC), Bio-GIC and liposome, 4) TGF-ß1-loaded-nanoparticles/scaffold, 5) Vascular endothelial growth factor (VEGF)-loaded-fiber and hydrogel, 6) TGF-ß1/VEGF-loaded-nanocrystalline calcium sulfate/hydroxyapatite/calcium sulfate, 7) Epidermal growth factor-loaded- nanosphere, 8) Stem cell factor/DSCs-loaded-hydrogel and Silk fibroin, 9) VEGF/BMP-2/DSCs-loaded-Three-dimensional matrix, 10) VEGF/DSCs-loaded-microsphere/hydrogel, and 11) BMP-2/DSCs and VEGF/DSCs-loaded-Collagen matrices. The included delivery systems showed viability, except for Bio-GIC on day 3. The choice of specific growth factors and delivery systems (<i>i.e.</i>, BMP-2-loaded-microsphere and VEGF-loaded-hydrogel) resulted in a greater gene expression.</p><p><strong>Conclusions: </strong>This study, with low-level evidence obtained from <i>ex vivo</i> studies, suggests that growth factor delivery systems induce cell proliferation, mineralization, and differentiation toward a therapeutic potential in regenerative endodontics.</p>\",\"PeriodicalId\":10979,\"journal\":{\"name\":\"Current stem cell research & therapy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current stem cell research & therapy\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2174/1574888X17666220609093939\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL & TISSUE ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current stem cell research & therapy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/1574888X17666220609093939","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}
引用次数: 0
摘要
目的目前的系统综述旨在提供评估牙科干细胞（DSC）和生长因子递送系统的生物学相互作用的可用离体证据。方法根据系统评价和荟萃分析的首选报告项目（PRISMA）指南，截至2022年1月，在电子数据库（PubMed/Medline、Scopus、Web of Science和Google Scholar）中进行了系统搜索。包括评估DSCs和生长因子递送系统的生物学相互作用的研究。结果指标为细胞的相容性、矿化和分化。结果选择了10项研究进行定性综合。以下生长因子递送系统表现出足够的细胞相容性、增强的矿化作用以及DSCs的成骨/成牙本质细胞分化潜力：1）负载成纤维细胞生长因子（FGF-2）的微球和丝素蛋白，2）负载骨形态发生蛋白-2（BMP-2）的微球以及介孔硅酸钙支架，3）转化生长因子Betha 1（TGF-ß1）负载微球、玻璃离聚物水泥（GIC）、生物-GIC和脂质体，4）TGF-ł1负载纳米颗粒/支架，5）血管内皮生长因子（VEGF）负载纤维和水凝胶，6）TGF-223; 1/VEGF负载纳米晶硫酸钙/羟基磷灰石/硫酸钙，7）表皮生长因子负载纳米球，8）干细胞因子/DSC负载的水凝胶和丝素蛋白，9）VEGF/BMP-2/DSC负载的三维基质，10）VEGF/DSC负载的微球/水凝胶，以及11）BMP-2/DSCs和VEGF/DSCs负载的胶原基质。除了第3天的Bio-GIC外，所包含的递送系统显示出生存能力。选择特定的生长因子和递送系统（即BMP-2负载的微球和VEGF负载的水凝胶）导致更大的基因表达。结论本研究从离体研究中获得了低水平的证据，表明生长因子递送系统诱导细胞增殖、矿化和分化，在再生牙髓病中具有治疗潜力。
The Role of Growth Factor Delivery Systems on Cellular Activities of Dental Stem Cells: A Systematic Review (Part II).
Objective: The current systematic review aims to provide the available ex vivo evidence evaluating the biological interactions of dental stem cells (DSCs) and growth factor delivery systems.
Methods: Following the Preferred Reporting Items for a Systematic Reviews and Meta-Analyses (PRISMA) guidelines, systematic search was conducted in the electronic databases (PubMed/Medline, Scopus, Web of Science, and Google Scholar) up to January 2022. Studies evaluating the biological interactions of DSCs and growth factor delivery systems were included. The outcome measures were cell cytocompatibility, mineralization, and differentiation.
Results: Sixteen studies were selected for the qualitative synthesis. The following growth factor delivery systems exhibit adequate cytocompatibility, enhanced mineralization, and osteo/odontoblast differentiation potential of DSCs: 1) Fibroblast growth factor (FGF-2)-loaded-microsphere and silk fibroin, 2) Bone morphogenic protein-2 (BMP-2)-loaded-microsphere and mesoporous calcium silicate scaffold, 3) Transforming growth factor Beta 1 (TGF-ß1)-loaded-microsphere, glass ionomer cement (GIC), Bio-GIC and liposome, 4) TGF-ß1-loaded-nanoparticles/scaffold, 5) Vascular endothelial growth factor (VEGF)-loaded-fiber and hydrogel, 6) TGF-ß1/VEGF-loaded-nanocrystalline calcium sulfate/hydroxyapatite/calcium sulfate, 7) Epidermal growth factor-loaded- nanosphere, 8) Stem cell factor/DSCs-loaded-hydrogel and Silk fibroin, 9) VEGF/BMP-2/DSCs-loaded-Three-dimensional matrix, 10) VEGF/DSCs-loaded-microsphere/hydrogel, and 11) BMP-2/DSCs and VEGF/DSCs-loaded-Collagen matrices. The included delivery systems showed viability, except for Bio-GIC on day 3. The choice of specific growth factors and delivery systems (i.e., BMP-2-loaded-microsphere and VEGF-loaded-hydrogel) resulted in a greater gene expression.
Conclusions: This study, with low-level evidence obtained from ex vivo studies, suggests that growth factor delivery systems induce cell proliferation, mineralization, and differentiation toward a therapeutic potential in regenerative endodontics.
期刊介绍：
Current Stem Cell Research & Therapy publishes high quality frontier reviews, drug clinical trial studies and guest edited issues on all aspects of basic research on stem cells and their uses in clinical therapy. The journal is essential reading for all researchers and clinicians involved in stem cells research.
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