用生物斑点法评价木霉®处理玉米种子的效果

G. M. Silva, L. Peixoto, A. Fujii, J. J. D. Parisi, R. H. Aguiar, Juliana A. Fracarolli
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引用次数: 5

摘要

本研究旨在研究生物保护剂哈兹木霉对玉米种子处理后即刻和贮藏期间的处理效果。通过萌发试验、活力试验(冷态试验、加速老化试验和气长试验)对玉米种子进行了分析,并通过生物斑点法验证了处理后种子的活力。建议进行种子处理,因为大约90%供人类和动物食用的作物是种子繁殖的。存在于种子中的病原体除了在其他地区引入病原体外,还会导致成体植物的疾病增加。因此,种子处理比化学药剂对植物防病效果更好。生物保护剂具有拮抗真菌,产生代谢物和酶,避免植物病原真菌的增殖。在生物保护剂中,用于种子植物检疫控制的木霉尤为突出。试验与处理一起进行,以评估生物保护剂处理引起的生理电位。生物斑点试验与卫生试验并行进行。发芽率分别为93.0%、87.5%和87.5%,均高于85%的商品化最低要求。通过对活力和卫生试验的分析,验证了最佳处理是在第0天。在生物斑点分析的种子中,利用激光斑点对比分析(LASCA)技术可以鉴定真菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Maize Seeds Treated with Trichodermil® through Biospeckle
The present work aimed to study the treatment with the bioprotector Trichoderma harzianum on maize seeds immediately after treatment and during storage. Maize seeds were analyzed through germination test, vigor tests (cold test, accelerated aging and aerial length), and the viability of the treated seeds was verified through the biospeckle. Seed treatment is recommended because approximately 90% of crops intended for human and animal feeding are seed-propagated. A pathogen present in the seed results in increased diseases in the adult plant, in addition to introducing pathogens in other areas. So the seed treatment can provide better results than chemicals disease control on plants. Bioprotectors have antagonistic fungi that produce metabolites and enzymes that will avoid the proliferation of phytopathogenic fungi. Among the bioprotectors, Trichoderma spp., used for phytosanitary control of seeds, stands out. The tests were carried out with treatment in order to evaluate the physiological potential caused by the treatment with the bioprotector. The biospeckle experimental runs were performed in parallel with the sanitation test. The germination percentages of 93.0%, 87.5%, and 87.5%, respectively, showed that they were all above the minimum requirement for commercialization, which is 85%. Analyzing the vigor and sanitation tests, it was verified that the best treatment was on day zero. Among the seeds analyzed through biospeckle, using the laser speckle contrast analysis (LASCA) technique, it was possible to identify fungi activity.
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