R. Azmy, E. G. E. El Gohary, D. Salem, M. Abdou, M. Salama, D. Mahmoud
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Biochemical and histopathological effect of the essential oil of Citrus sinensis (L.) Osbeck on larvae of Culex pipiens Linnaeus, 1758 (Diptera: Culicidae)
Abstract In this study, the third instar larvae of Culex pipiens Linnaeus, 1758 were treated with a lethal concentration of the essential oil (EO) of Citrus sinensis (L.) Osbeck; its nanoemulsion and the EO dissolved in the absolute ethanol were applied. The tested EO induced biochemical and histological changes in the treated larvae. The biochemical changes were presented by an increase in the levels of the acid phosphatase and alkaline phosphatase and non-significant change in the activity of acetylcholinesterase and glutathione S-transferase. Histological studies revealed obvious alterations in the cuticle, muscles, and midgut tissues including changes on the ultrastructural level.
期刊介绍:
Aquatic Insects is an international journal publishing original research on the systematics, biology, and ecology of aquatic and semi-aquatic insects.
The subject of the research is aquatic and semi-aquatic insects, comprising taxa of four primary orders, the Ephemeroptera, Odonata, Plecoptera, and Trichoptera but also aquatic and semi-aquatic families of Hemiptera, Coleoptera, and Diptera, as well as specific representatives of Hymenoptera , Lepidoptera, Mecoptera, Megaloptera , and Neuroptera that occur in lotic and lentic habitats during part of their life cycle. Studies on other aquatic Hexapoda (i.e., Collembola) will be only accepted if space permits. Papers on other aquatic Arthropoda (e.g., Crustacea) will not be considered, except for those closely related to aquatic and semi-aquatic insects (e.g., water mites as insect parasites).
The topic of the research may include a wide range of biological fields. Taxonomic revisions and descriptions of individual species will be accepted especially if additional information is included on habitat preferences, species co-existing, behavior, phenology, collecting methods, etc., that are of general interest to an international readership. Descriptions based on single specimens are discouraged.
Detailed studies on morphology, physiology, behavior, and phenology of aquatic insects in all stadia of their life cycle are welcome as well as the papers with molecular and phylogenetic analyses, especially if they discuss evolutionary processes of the biological, ecological, and faunistic formation of the group.