揭示单个狂犬病毒颗粒进入细胞的动力学机制

IF 3.1 4区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Research in Chinese Universities Pub Date : 2022-01-01 Epub Date: 2022-05-02 DOI:10.1007/s40242-022-2069-y

Siying Li, Yangang Pan, Honggang Teng, Yuping Shan, Guocheng Yang, Hongda Wang

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引用次数: 0

摘要

狂犬病病毒是一种神经性病毒，可导致人类和动物患上致命疾病。尽管研究单个狂犬病毒与细胞膜之间的相互作用对于了解其致病机理十分必要，但单个狂犬病毒在活细胞中的内化动态机制在很大程度上仍然难以捉摸。在此，我们利用基于原子力显微镜（AFM）的新型力追踪技术记录了单个病毒进入宿主细胞的过程。我们发现，狂犬病毒内化的力分布在（65±25） pN，时间上有两个峰值，间隔分别为（237.2±59.1）和（790.3±134.4） ms，相应的速度分别为 0.12 和 0.04 µm/s。我们的研究结果让我们深入了解了内吞过程中病毒形状的影响。这份报告对于理解狂犬病毒早期感染的动态机制将很有意义：补充材料见本文在线版 10.1007/s40242-022-2069-y。

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Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle.

The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 µm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.

Electronic supplementary material: Supplementary material is available in the online version of this article at 10.1007/s40242-022-2069-y.

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来源期刊

Chemical Research in Chinese Universities 化学-化学综合

CiteScore

5.30

自引率

6.50%

发文量

152

审稿时长

3.0 months

期刊介绍： The journal publishes research articles, letters/communications and reviews written by faculty members, researchers and postgraduates in universities, colleges and research institutes all over China and overseas. It reports the latest and most creative results of important fundamental research in all aspects of chemistry and of developments with significant consequences across subdisciplines. Main research areas include (but are not limited to): Organic chemistry (synthesis, characterization, and application); Inorganic chemistry (bio-inorganic chemistry, inorganic material chemistry); Analytical chemistry (especially chemometrics and the application of instrumental analysis and spectroscopy); Physical chemistry (mechanisms, catalysis, thermodynamics and dynamics); Polymer chemistry and polymer physics (mechanisms, material, catalysis, thermodynamics and dynamics); Quantum chemistry (quantum mechanical theory, quantum partition function, quantum statistical mechanics); Biochemistry; Biochemical engineering; Medicinal chemistry; Nanoscience (nanochemistry, nanomaterials).