不同提取方法及GC-MS对山竹果皮代谢组学分析

Q3 Agricultural and Biological Sciences
Siti Farah Mamat, Kamalrul Azlan Azizan, S. Baharum, N. Noor, W. Aizat
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引用次数: 12

摘要

山竹藤。据报道,山竹果含有几种生物活性代谢物,已用于各种传统疾病治疗、营养补充剂和护肤产品。然而,详细描述其特性和代谢物含量的报道仍然很少。大多数早期的报道只关注特定代谢物的提取,如山酮和花青素,而不是全面的代谢组谱。本研究采用全球代谢组学方法,采用气相色谱-质谱(GC-MS)分析方法,对成熟最后阶段(深紫色阶段)收获的山竹果皮的代谢物含量进行了分析。比较了五种不同的提取方法,不同的溶剂混合物,溶剂比,有或没有超声,旨在最大限度地从山竹组织中检测代谢物。结果表明,该方法使用甲醇/氯仿/水(3:1:1 v/v比)的混合溶剂与超声相结合,在生物重复中具有一致的再现性,并且与其他方法相比,成功地获得了最多的代谢物。综合不同提取方法的结果,初步鉴定出73种代谢物,包括糖(49.32%)、醇(9.59%)、糖酸(8.22%)、有机酸(6.85%)、酚酸(5.48%)、芳香族化合物(2.74%)和醛(1.37%)。这一发现提供了山竹果皮代谢物含量的概述,并对这种有益果实的非靶向代谢组学方法的提取方法进行了全面评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomics analysis of mangosteen (Garcinia mangostana Linn.) fruit pericarp using different extraction methods and GC-MS
Garcinia mangostana Linn. (mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products. However, reports detailing its characterization and metabolite content are still scarce. Most of the earlier reports only focused on the extraction of specific metabolites such as xanthones and anthocyanins, rather than a comprehensive metabolome profile. In this study, global metabolomics approach using gas chromatography-mass spectrometry (GC-MS) analysis was conducted to profile the metabolite content of mangosteen fruit pericarp harvested at the final stage of ripening (dark purple stage). Five different extraction methods, which vary in their solvent mixtures, solvent ratios, with or without sonication were compared, aimed to maximize metabolite detection from the mangosteen tissue. The results showed that the method using a combined solvent mixture of methanol/chloroform/water (3:1:1 v/v ratio) with sonication has consistent reproducibility amongst biological replicates and successfully yielded the highest number of metabolites compared to the other methods. By combining the results of different extraction methods, we tentatively identified a total of 73 metabolites comprising of sugars (49.32%), alcohols (9.59%), sugar acids (8.22%), organic acids (6.85%), phenolic acids (5.48%), aromatic compounds (2.74%) and aldehyde (1.37%). This finding provides an overview of the metabolite content of mangosteen pericarp and comprehensive assessment of extraction methods for an untargeted metabolomics approach of this beneficial fruit.
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来源期刊
Plant Omics
Plant Omics 生物-植物科学
CiteScore
1.30
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.
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