MAPK信号通路基因的激活导致高温适应性barkeri新绥螨的耐热性增强(Hughes)

IF 1.3 3区 农林科学 Q2 ENTOMOLOGY
Chuanbei Tian, Guangyun Li, Mian Wang, Hanqiu Chen, Yishu Ding, Gang Chen, YA-YING Li, Yuzhen Nima, Huai Liu
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引用次数: 0

摘要

摘要捕食性螨是蔬菜上植食性螨和几种小害虫的重要生物防治剂,但其防治效果往往受到环境变化或波动的限制,特别是在全球变暖的威胁下。为了减轻高温环境的不良影响,通过长期热驯化(35°C)和定期热硬化(45°C),从常规菌株(CS)中筛选出了barkeri新绥螨(Neoseiulus barkeri, Hughes)的高温适应菌株(HTAS)。为了探讨巴氏螨耐热性增强的分子机制,本研究从巴氏螨中鉴定了4个丝裂原活化蛋白激酶(MAPK)通路基因。在不同发育阶段的表达水平显示,4个MAPK通路基因在成虫中表达量最高,在卵和若虫中表达量最低。在42℃高温胁迫下,两株巴氏奈氏菌中4个MAPK通路基因的表达量在短时间内迅速升高,且在HTAS巴氏奈氏菌中的表达量增加幅度更为明显。此外,暴露时间延长4 h后,CS barkeri的MAPK通路基因表达量急剧下降,而HTAS barkeri的MAPK通路表达量在4 h后仍保持升高。这些结果表明,MAPK信号通路参与了捕食性植物螨耐热性的形成,为提高多变环境下捕食性螨的生物防治效率提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Activation of MAPK signaling pathway genes leads to promoted thermotolerance in a high-temperature adapted predatory mite Neoseiulus barkeri (Hughes)
Abstract Predatory mites are important biological control agents for phytophagous mites and several small insect pests on vegetables, while the control efficiency is often limited by the changeable or fluctuating environment, especially under the threat of global warming. To mitigate the undesired impacts of high temperature environment, a high-temperature adapted strain (HTAS) of the predatory mite Neoseiulus barkeri (Hughes) was screened from a conventional strain (CS) by long-term heat acclimation (35°C) and regular heat hardenings (45°C). In this study, to investigate the molecular mechanisms of enhanced thermotolerance in HTAS N. barkeri, four mitogen-activated protein kinase (MAPK) pathway genes were identified from the predatory mite N. barkeri. The expression levels at different developmental stages showed four MAPK pathway genes were highest at adults and lowest at eggs or nymphs in both strains. Under 42°C high temperature stress, the expression levels of four identified MAPK pathway genes increased rapidly at a short time in both N. barkeri strains and the magnitude of expression increase in HTAS N. barkeri was even more evident. In addition, the MAPK pathway genes expression in CS N. barkeri sharply decreased following longer exposure periods at 4 h, whereas the expression in HTAS N. barkeri remained elevated after 4 h. These results suggested that MAPK signaling pathway participated in the formation of thermotolerance in predatory phytoseiid mites, which provided new insights in promoting the biological control efficiency of predatory mites under changeable environment.
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来源期刊
CiteScore
2.20
自引率
33.30%
发文量
152
期刊介绍: Systematic and Applied Acarology (SAA) is an international journal of the Systematic and Applied Acarology Society (SAAS). The journal is intended as a publication outlet for all acarologists in the world. There is no page charge for publishing in SAA. If the authors have funds to publish, they can pay US$20 per page to enable their papers published for open access. SAA publishes papers reporting results of original research on any aspects of mites and ticks. Due to the recent increase in submissions, SAA editors will be more selective in manuscript evaluation: (1) encouraging more high quality non-taxonomic papers to address the balance between taxonomic and non-taxonomic papers, and (2) discouraging single species description (see new special issues for single new species description) while giving priority to high quality systematic papers on comparative treatments and revisions of multiple taxa. In addition to review papers and research articles (over 4 printed pages), we welcome short correspondence (up to 4 printed pages) for condensed version of short papers, comments on other papers, data papers (with one table or figure) and short reviews or opinion pieces. The correspondence format will save space by omitting the abstract, key words, and major headings such as Introduction.
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