HPLC-UV法测定人血浆中文拉法辛及其代谢产物邻去甲基文拉法辛的含量

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
T. Pawiński, I. Szlaska, Monika Kukiełka, Kinga Kośnik
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引用次数: 0

摘要

本文介绍了一种简单、快速、经济高效的HPLC-UV法测定人血浆中文拉法辛及其活性代谢产物O-去甲基文拉法辛的含量。样品制备基于液-液萃取程序,萃取时间短。以盐酸普唑嗪为内标。HPLC分离在Supelcosil LC-CN柱上进行,使用由乙腈:25mM磷酸盐缓冲液组成的流动相,pH为3,5(15:85v/v)。在25-1000 ng/mL的浓度范围内,校准曲线是线性的,这适用于每天37.5 mg至375.0 mg给药后盐酸文拉法辛的药代动力学研究。该方法根据国际指南进行了充分验证,
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validated HPLC-UV method for determination of venlafaxine and its metabolite - o-desmethylvenlafaxine in human plasma
This paper describes a simple, rapid and cost-effective HPLC-UV method for the determination of venlafaxine and its active metabolite O-desmethylvenlafaxine in human plasma. Sample preparation is based on a liquid-liquid extraction procedure with a short extraction time. Prazosine hydrochloride was used as the internal standard. The HPLC separation was performed on a Supelcosil LC-CN column, using a mobile phase consisting acetonitrile : 25 mM phosphate buffer at pH 3,5 (15:85 v/v). The calibration curve is linear in the concentrations range of 25-1000 ng/mL, which is suitable for pharmacokinetic studies of venlafaxine hydrochloride following of dosing from 37.5 mg to 375.0 mg per day. The method was fully validated according to the international guidances,
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
74
审稿时长
6-12 weeks
期刊介绍: The international journal of the Polish Pharmaceutical Society is published in 6 issues a year. The journal offers Open Access publication of original research papers, short communications and reviews written in English, in all areas of pharmaceutical sciences. The following areas of pharmaceutical sciences are covered: Analysis, Biopharmacy, Drug Biochemistry, Drug Synthesis, Natural Drugs, Pharmaceutical Technology, Pharmacology and General. A bimonthly appearing in English since 1994, which continues “Acta Poloniae Pharmaceutica”, whose first issue appeared in December 1937. The war halted the activity of the journal’s creators. Issuance of “Acta Poloniae Pharmaceutica” was resumed in 1947. From 1947 the journal appeared irregularly, initially as a quarterly, then a bimonthly. In the years 1963 – 1973 alongside the Polish version appeared the English edition of the journal. Starting from 1974 only works in English are published in the journal. Since 1995 the journal has been appearing very regularly in two-month intervals (six books a year). The journal publishes original works from all fields of pharmacy, summaries of postdoctoral dissertations and laboratory notes.
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