短发夹RNA介导的人呼吸道合胞病毒基质基因沉默作为一种有效的抗病毒策略

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-05-16 DOI:10.2217/fvl-2022-0207

Saeid Amiri Zadeh Fard, Haniyeh Abuei, A. Farhadi, Gholamreza Rafiei Dehbidi, F. Zare, Zahra Abbasfard, A. Behbahani

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引用次数: 0

摘要

目的:人呼吸道合胞病毒(HRSV)是呼吸道感染的常见原因，特别是在婴儿和老年人中。利巴韦林是美国fda批准的唯一一种治疗HRSV感染的抗病毒药物，但是它有副作用。方法:我们设计了一种靶向HRSV- M基因的shRNA来对抗HRSV的复制。结果:结果表明shRNA与利巴韦林具有相似的显著的病毒载量降低(99.8%)。此外，与单独使用利巴韦林或M-shRNA相比，利巴韦林和M-shRNA联合治疗可显著降低病毒载量。结论:这些结果提示M-shRNA可能是一种潜在的新的HRSV复制抑制剂，并可能在未来为HRSV感染提供更安全、更有效的治疗选择。

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Short hairpin RNA-mediated matrix gene silencing of human respiratory syncytial virus as a potent antiviral strategy

Aim: Human respiratory syncytial virus (HRSV) is a common cause of respiratory infections, particularly in infants and the elderly. Ribavirin is the only US FDA-approved antiviral drug for HRSV infection, but it has unwanted side effects. Methods: We engineered an shRNA targeting the HRSV- M gene to antagonize HRSV replication. Results: The results showed that shRNA had a similarly significant reduction in viral load (99.8%) as ribavirin. In addition, combined treatment with ribavirin and M-shRNA resulted in a significant reduction in viral load compared with ribavirin or M-shRNA alone. Conclusion: These results suggest that M-shRNA could be a potential new inhibitor of HRSV replication and could offer a safer and more effective treatment option for HRSV infection in the future.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.