痛风发作期患者血清IL-1β和IL-18浓度与心血管改变无关

Q3 Medicine
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A total of 83 consecutive patients were included: 18 with inactivated osteoarthritis, 29 with asymptomatic hyperuricemia, 22 gouty arthritis without tophi and 18 gouty tophi individuals out of flare. Serum interleukin concentrations were determined by enzyme-linked immunosorbent assay (ELISA) with Human IL-1β and IL-18 ELISA kits (Platinum, eBioscience, Vienna, Austria). By applying ultrasound were measured: renal resistive index (RRI) with 3.5 MHz transducer working with pulse Doppler frequency of 2.5 MHz and left ventricular mass index (LVMi), determined with 2.5 MHz transducer Phased Array. Data were analyzed by One-Sample Kolmogorov-Smirnov, ANOVA, Tukey HSD, Kruskal Wallis, Mann-Whitney and Fisher’s exact test. Correlational analyzes were performed by using the Spearman correlation coefficient. Results: In gouty tophi subjects serum IL-1β level was undetectable compared to the other three groups, (p<0.001). 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引用次数: 0

摘要

背景:文献中有报道称,引发和维持痛风炎症的关键白细胞介素IL-1β和IL-18与肾脏和心血管疾病有关。它们在先天免疫反应和血管病理学中具有主要的调节功能。目的:我们旨在测定失活骨关节炎对照组、无症状高尿酸血症患者、无痛风性关节炎受试者和痛风性痛风患者的血清IL-1β和IL-18水平,并确定其血清浓度是否与肾脏和心脏的超声改变有关。材料和方法:本研究采用横断面设计。共有83名连续患者被纳入:18名失活骨关节炎患者,29名无症状高尿酸血症患者,22名无痛风性关节炎患者和18名痛风性痛风患者。使用人IL-1β和IL-18 ELISA试剂盒(Platinum,eBioscience,Vienna,Austria)通过酶联免疫吸附试验(ELISA)测定血清白细胞介素浓度。通过应用超声测量:用3.5MHz换能器工作,脉冲多普勒频率为2.5MHz的肾电阻指数(RRI)和用2.5MHz换能器相控阵测定的左心室质量指数(LVMi)。数据采用单样本Kolmogorov-Smirnov、ANOVA、Tukey HSD、Kruskal-Wallis、Mann-Whitney和Fisher精确检验进行分析。使用Spearman相关系数进行相关分析。结果:与其他三组相比,痛风患者的血清IL-1β水平未检测到(p<0.001)。各组的血清IL-18浓度可比较(p=0.154)。鉴于痛风患者的IL-1β含量未检测到,未对该组与血清尿酸浓度、RRI和LVMi进行相关性分析。在IL-1β值高于零的其余三组中,我们没有检测到与上述参数的相关性。IL-18浓度与血清尿酸、RRI和LVMi无相关性。结论:我们认为血清IL-1β和IL-18水平不能反映痛风患者的疾病严重程度和心血管风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum concentrations of IL-1β and IL-18 in gout patients out of flare are not connected to cardiovascular alterations
Background: In the literature there are reports that the key interleukins, IL-1β and IL-18, for the initiation and maintenance of gouty inflammation are associated with renal and cardiovascular disorders. They have a major regulatory function in the innate immune response and in vascular pathology. Objective: We aimed to determine serum levels of IL-1β and IL-18 in controls with inactivated osteoarthritis, patients with asymptomatic hyperuricemia, gouty arthritis without tophi subjects and gouty tophi individuals out of flare, and to establish whether their serum concentrations are connected to ultrasound alterations of the kidneys and heart. Materials and methods: The study is cross-sectional in design. A total of 83 consecutive patients were included: 18 with inactivated osteoarthritis, 29 with asymptomatic hyperuricemia, 22 gouty arthritis without tophi and 18 gouty tophi individuals out of flare. Serum interleukin concentrations were determined by enzyme-linked immunosorbent assay (ELISA) with Human IL-1β and IL-18 ELISA kits (Platinum, eBioscience, Vienna, Austria). By applying ultrasound were measured: renal resistive index (RRI) with 3.5 MHz transducer working with pulse Doppler frequency of 2.5 MHz and left ventricular mass index (LVMi), determined with 2.5 MHz transducer Phased Array. Data were analyzed by One-Sample Kolmogorov-Smirnov, ANOVA, Tukey HSD, Kruskal Wallis, Mann-Whitney and Fisher’s exact test. Correlational analyzes were performed by using the Spearman correlation coefficient. Results: In gouty tophi subjects serum IL-1β level was undetectable compared to the other three groups, (p<0.001). The serum concentration of IL-18 was comparable across the groups, (p=0.154). Given that the level of IL-1β was undetectable in gouty tophi patients, a correlation analysis in this group with serum uric acid concentration, RRI and LVMi was not performed. In the remaining three groups, which had values of IL-1β above zero, we did not detect an association with the above mentioned parameters. No correlation was found between IL-18 concentration and serum uric acid, RRI and LVMi in the groups. Conclusion: We suggest that serum IL-1β and IL-18 levels do not reflect the severity of the disease and cardiovascular risk in the examined gout patients.
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来源期刊
Revmatologiia (Bulgaria)
Revmatologiia (Bulgaria) Medicine-Rheumatology
CiteScore
0.30
自引率
0.00%
发文量
21
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