印度西高止山脉特有蛙肯氏夜蛾微卫星标记的开发、鉴定及与其他夜蛾物种的交叉扩增

IF 0.7 4区生物学 Q4 ZOOLOGY

Current Herpetology Pub Date : 2020-08-28 DOI:10.5358/hsj.39.196

R. Srirama, H. Priti, N. A. Aravind, G. Ravikanth

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引用次数: 1

摘要

摘要/ Abstract摘要:Nyctibatrachus属是印度西高止山脉的特有种，包括许多分布受限的种。kpholeynyctibatrachus kempholeyensis是分布在中西部高止山脉的特有物种。本文描述了8个多态微卫星标记的开发。这些标记在其他夜莺种中进行了交叉扩增试验。此外，我们还证实了来自另一个物种N. major的3个微卫星标记的交叉扩增。对11个微卫星进行筛选，发现每个位点有3 ~ 8个等位基因，杂合度在0.15 ~ 0.95之间。这些微卫星标记可用于研究破碎生境的种群多样性，量化遗传结构，评估该特有属种群间的基因流动。

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Development and Characterization of Microsatellite Markers for the Endemic Frog Nyctibatrachus kempholeyensis and Cross Amplification with Other Nyctibatrachus Species from the Western Ghats, India

Abstract: The genus Nyctibatrachus is endemic to the Western Ghats, India, and includes a number of species that are highly restricted in their distribution. Nyctibatrachus kempholeyensis is an endemic species distributed in the Central Western Ghats. In this paper we describe the development of eight polymorphic microsatellite markers for N. kempholeyensis. These markers were tested for cross-amplification in other Nyctibatrachus species. In addition, we confirmed the cross-amplification of three microsatellite markers from another species, N. major. The 11 microsatellites were screened with 76 individuals of N. kempholeyensis and were found to have 3–8 alleles per locus, with observed heterozygosity ranging from 0.15 to 0.95. These microsatellite markers could be used to study population diversity across fragmented habitats, to quantify genetic structure, and to assess gene flow across the populations in this endemic genus.

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来源期刊

Current Herpetology Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

1.20

自引率

14.30%

发文量

期刊介绍： Current Herpetology publishes original research articles on amphibians and reptiles. It is the official journal of the Herpetological Society of Japan and is a continuation of Acta Herpetologica Japonica (1964–1971) and Japanese Journal of Herpetology (1972-1999).