载百里香酚壳聚糖纳米复合材料对致龋细菌的抗菌及细胞毒性研究

Q3 Pharmacology, Toxicology and Pharmaceutics

Journal of HerbMed Pharmacology Pub Date : 2023-03-18 DOI:10.34172/jhp.2023.23

Matin Kordestani, M. Rashidipour, H. Mahmoudvand, Shoukofeh Jalali, F. Kooshki

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引用次数: 0

摘要

简介：蛀牙是世界上最常见的营养性传染病。本工作旨在研究胸腺素负载的壳聚糖纳米复合材料（TLCN）对变形链球菌和粘放线菌作为主要致龋细菌的抗菌作用。方法：用TLCN对变形链球菌和粘球菌进行抗菌活性测定。分别用Bradford法和细胞活力测定法研究了对受试细菌蛋白质渗漏的影响及其细胞毒性。结果：纳米复合材料的尺寸在100～600nm之间。据报道，与纳米复合材料+氯己定相关的两种细菌的最佳最小抑制浓度（MIC）均为2.66。TLCN剂量依赖性地增加蛋白渗漏（P＜0.05），纳米复合物对正常细胞（HGF1-PI1）和癌症细胞（KB）的50%细胞毒浓度（CC50）分别为149.6和68.4μg/mL。结论：TLCN，特别是与氯己定联合使用，对主要致龋细菌具有较强的抗菌作用。然而，主要在临床环境中，还需要进行其他检查来阐明确切的机制及其毒性。

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Antibacterial and cytotoxicity of chitosan nanocomposite loaded with thymol against some cariogenic bacteria

Introduction: Tooth decay as the most common infectious-nutritional disease in the world. The current work aims to investigate the antibacterial effect of thymol-loaded chitosan nanocomposite (TLCN) against Streptococcus mutans and Actinomyces viscosus as the main cariogenic bacteria. Methods: Antibacterial activity of TLCN was assessed on S. mutans and A. viscosus. The effects on protein leakage in the tested bacteria, as well as its cytotoxicity, were studied by Bradford’s method and cell viability assay, respectively. Results: The size of the nanocomposite varied from 100 to 600 nm. The best minimum inhibitory concentration (MIC) related to nanocomposite + chlorhexidine was reported 2.66 for both bacteria. TLCN dose-dependently increased the protein leakage (P < 0.05). The 50% cytotoxic concentration (CC50) of nanocomposite against on normal (HGF1-PI1) and cancer (KB) cells were 149.6 and 68.4 μg/mL, respectively. Conclusion: TLCN, especially in combination with chlorhexidine, displayed potent antibacterial effects against the main cariogenic bacterial causes. Nevertheless, other examinations are required to illuminate the precise mechanisms and its toxicity mainly in clinical settings.

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来源期刊

Journal of HerbMed Pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Journal of Herbmed Pharmacology (J Herbmed Pharmacol) is the intersection between medicinal plants and pharmacology. This international journal publishes manuscripts in the fields of medicinal plants, pharmacology and therapeutic. This journal aims to reach all relevant national and international medical institutions and persons in electronic version free of charge. J Herbmed Pharmacol has pursued this aim through publishing editorials, original research articles, reviews, mini-reviews, commentaries, letters to the editor, hypothesis, case reports, epidemiology and prevention, news and views. In this journal, particular emphasis is given to research, both experimental and clinical, aimed at protection/prevention of diseases. A further aim of this journal is to emphasize and strengthen the link between herbalists and pharmacologists. In addition, J Herbmed Pharmacol welcomes basic biomedical as well as pharmaceutical scientific research applied to clinical pharmacology. Contributions in any of these formats are invited for editorial consideration following peer review by at least two experts in the field.