新型心肌灌注显像示踪剂99Tcm-3SPboroxime SPECT显像的实验研究

中华核医学与分子影像杂志 Pub Date : 2020-02-25 DOI:10.3760/CMA.J.ISSN.2095-2848.2020.02.002

Xiao-Ying Xi, Zongyao Zhang, Lixia Zhang, Kai Han, Zuoquan Zhao

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引用次数: 0

摘要

目的评价99Tcm-3SP作为快速心肌灌注显像（MPI）示踪剂在正常和急性心肌梗死（AMI）小型猪中的优越性。方法制备99Tcm-3SP和99Tcm-Teboroxine（简称99Tcm-TEBO）。在2头健康迷你猪中分别静脉注射约370MBq99Tcm-3SP或99Tcm-TEBO。注射后立即采集动态平面图像，并使用标准SPECT相机持续20分钟。测量心脏、肝脏和肺部的放射性吸收，并计算心脏/肝脏和心脏/肺部随时间的比值。使用碲化镉锌SPECT（CZT-SPECT）对4头正常猪和1头AMI猪进行了动态SPECT研究。立即开始列表模式采集，并在静脉注射约370MBq 99Tcm-TEBO和99Tcm-3SP后持续15分钟。两种放射性示踪剂在同一头猪体内的注射在2天内完成。测量心脏和肝脏的放射性吸收，并计算心脏/肝脏比率。还对图像质量进行了评估。采用配对t检验对数据进行分析。结果99Tcm-TEBO和99Tcm-3SP的放射化学纯度均在95%以上。99Tcm-3SP的初始心脏摄取与99Tcm-TEBO非常接近（平面图像，注射后2分钟：309.32×103vs 314.13×103计数/MBq；SPECT图像，注射2分钟（校正）：7.96±0.87 vs 8.24±1.53，t=0.277，P>0.05），但心肌滞留时间远长于99Tcm-TEBO（平面图，注射后20min:21.867×103vs143.19×103计数/MBq；SPECT图像，注射后15min（校正）：6.76±0.45 vs 5.06±0.33，t=-12.412，P=0.001）。SPECT MPI图像显示示踪剂分布均匀，在正常心肌中具有清晰可见的心肌边界，在梗死心肌中具有强烈的灌注缺陷。在正常猪和AMI猪中注射99Tcm-3SP后的前15分钟内，可以在5分钟成像窗口中的任何一个中获得高质量SPECT图像。结论99Tcm-3SP是一种有前途的99Tcm标记的快速MPI放射性示踪剂，因为它具有高的心脏摄取、长的心肌滞留时间（注射后20min）和优越的SPECT图像质量。关键词：心肌灌注成像；氧化物；硼酸；锝；层析成像，发射计算机，单光子；猪

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Experimental study of SPECT imaging with novel myocardial perfusion imaging tracer: 99Tcm-3SPboroxime

Objective To assess the superiority of 99Tcm-3SPboroxime (99Tcm-3SP for short) as a fast-myocardial perfusion imaging (MPI) tracer in normal and acute myocardial infarction (AMI) mini-swine. Methods 99Tcm-3SP and 99Tcm-Teboroxime (99Tcm-TEBO for short) were prepared. Approximately 370 MBq 99Tcm-3SP or 99Tcm-TEBO was injected intravenously in 2 healthy mini-swine separately. Dynamic planar images were acquired immediately after injection and continued for 20 min using a standard SPECT camera. The radioactivity uptakes in the heart, liver, and lungs were measured, and heart/liver and heart/lung ratios over time were calculated. Dynamic SPECT studies were performed in 4 normal swine and 1 AMI-swine using cadmium zinc telluride-SPECT (CZT-SPECT). List mode acquisitions were immediately started and continued for 15 min after intravenous injection of approximately 370 MBq 99Tcm-TEBO and 99Tcm-3SP. The injection of two radiotracers in the same swine was completed within 2 d. The radioactivity uptakes in heart and liver were measured, and heart/liver ratio was calculated. Image quality was also evaluated. Paired t test was used to analyze the data. Results The radiochemical purity of 99Tcm-TEBO or 99Tcm-3SP were both above 95%. The initial heart uptake of 99Tcm-3SP was very close to that of 99Tcm-TEBO (planar image, 2 min postinjection: 309.32×103vs 314.13×103 counts/MBq; SPECT image, 2 min postinjection (corrected): 7.96±0.87 vs 8.24±1.53, t=0.277, P>0.05), but the myocardial retention time was much longer than that of 99Tcm-TEBO (planar image, 20 min postinjection: 218.67×103vs 143.19×103 counts/MBq; SPECT image, 15 min postinjection (corrected): 6.76±0.45 vs 5.06±0.33, t=-12.412, P=0.001). The uptake of liver and heart/liver ratio between 99Tcm-TEBO and 99Tcm-3SP were similar (t values: -1.332-1.101, all P>0.05 within 15 min). SPECT MPI images demonstrated uniform tracer distribution with clearly visualizable myocardial boundary in normal myocardium and intense perfusion defect in infarct myocardium. High quality SPECT images could be obtained in any of the 5 min imaging windows over the first 15 min after injection of 99Tcm-3SP in normal swine and AMI-swine. Conclusion 99Tcm-3SP is a promising 99Tcm-labeled radiotracer for fast-MPI considering its high heart uptake, long myocardial retention time (20 min postinjection) and superior SPECT image quality. Key words: Myocardial perfusion imaging; Oximes; Boronic acids; Technetium; Tomography, emission-computed, single-photon; Swine

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来源期刊

中华核医学与分子影像杂志核医学，分子影像

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期刊介绍： Chinese Journal of Nuclear Medicine and Molecular Imaging (CJNMMI) was established in 1981, with the name of Chinese Journal of Nuclear Medicine, and renamed in 2012. As the specialized periodical in the domain of nuclear medicine in China, the aim of Chinese Journal of Nuclear Medicine and Molecular Imaging is to develop nuclear medicine sciences, push forward nuclear medicine education and basic construction, foster qualified personnel training and academic exchanges, and popularize related knowledge and raising public awareness. Topics of interest for Chinese Journal of Nuclear Medicine and Molecular Imaging include: -Research and commentary on nuclear medicine and molecular imaging with significant implications for disease diagnosis and treatment -Investigative studies of heart, brain imaging and tumor positioning -Perspectives and reviews on research topics that discuss the implications of findings from the basic science and clinical practice of nuclear medicine and molecular imaging - Nuclear medicine education and personnel training - Topics of interest for nuclear medicine and molecular imaging include subject coverage diseases such as cardiovascular diseases, cancer, Alzheimer’s disease, and Parkinson’s disease, and also radionuclide therapy, radiomics, molecular probes and related translational research.